Chronic Restraint Stress Model
Studies have shown that sustained stress is a significant contributor to depression and that the main cause of depression is from chronic, low-intensity, long-term daily stress. Restraint stress is one of the widely used animal models for simulating stress in human life.
Our company has extensive experience in stress modeling and behavioral assessment. We specialize in providing our clients with appropriate animal models for simulating human depression and other psychiatric disorders and evaluating them using a variety of classical behavioral methods. We help our clients worldwide to have reliable and accurate results in the study of psychiatric disorders and their drug development process.
Introduction of The Chronic Restraint Stress Test
Chronic restraint affects a variety of behavioral and biological indicators in animals, including the development of mood disorders such as depression or anxiety. Chronic restraint stress is a commonly used stress model for non-invasive stimuli. During chronic restraint stress experiments, rodents are repeatedly placed in restraints, and by restricting their freedom of movement for a period of time, the animals experience depression-like symptoms such as weight loss, decreased appetite, and loss of pleasure. These symptoms can be improved with antidepressants.
Experimental Flow
The chronic restraint model produces pure psychological stress in rodents by means of behavioral restriction. The experiments were performed in an animal behavioral restrainer. During the experiment, the behavioral restraint was placed flat with the bottom open, so that the animal faced the behavioral restraint opening and entered the interior. The opening is closed and secured so that the rodent's range of motion in the restraint is less than its body length, limb movement is limited except for the head, which is free to move.
Fig. 1 Chronic restraint experimental model.
Our company offers flexible approaches to chronic restraint modeling, using either rats or mice for depression modeling, depending on our customers' research objectives.
| Moulding Method | |
|---|---|
| Times of evasion failures | The total number of times the animal did not complete the avoidance response within the set duration of the unconditioned stimulus. |
| Escape latency | The time of the 1st shuttle of the animal to the opposite side of the test chamber. |
| Rats |
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| Mice |
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Model Analysis Indicators
After the chronic restraint reaches the specified time, we will select the appropriate behavioral test method for the customer to analyze the model according to the specific experimental requirements of our customer. The behavioral analysis tests we offered include but are not limited to
- Weight test.
- Sucrose Preference Test.
- Novelty experiments.
Features of The Chronic Restraint Stress Experimental Model
- It belongs to the stress model of inescapability or coercive psychology, which can effectively induce typical stress responses.
- The pathogenic process of human physical and mental diseases has great similarity, and is one of the most widely used animal models of depression.
- For the study of the pathophysiology of depression has important implications for model building indicator tests.
Our company offers professional one-stop services from experimental design to results analysis to bring breakthroughs to your research. We guarantee on-time delivery of test results. If you need, you can contact us at any time.