Foot Volume Measurement
Foot volume measurements are widely used in researches related to the screening and identification of anti-inflammatory immune drugs.
Our company is committed to providing clients with accurate and reliable test results based on our extensive testing experience and advanced instrumentation, helping customers to be more efficient and time-saving in anti-inflammatory drug screening.
Introduction of The Foot Volume Measurement
Paw swelling in rats or mice is a key indicator for evaluating models of acute nonspecific inflammation. The test is primarily instrumented to measure the degree of paw swelling in rodents before and after drug administration. Our device evaluates and studies the effect, duration of action and efficacy pattern of anti-inflammatory drugs by accurately measuring and timing the process of swelling in rodent paws after inflammation and swelling.
Example of Application of Foot Volume Measurement
The foot volume measurement is a classic test for the evaluation of the activity of anti-inflammatory drugs. The experiment described below is a study of the anti-inflammatory activity of the aqueous and methanol extracts from the bulbils of Dioscorea bulbifera var Sativa. We hope this example will help our clients determine if foot volume measurement is appropriate for their study.
- Rats were labeled in groups, and initial paw swelling was tested and recorded.
- Test animals were injected orally with aqueous or methanolic extracts of Dioscorea bulbifera var Sativa at doses of 300 and 600 mg/kg.
- One hour after administration, 0.1 mL of carrageenan (1% in 0.9% NaCl) was injected into the subcutaneous tissue of the plantar aspect of the right hind paw of rats to induce oedema.
- Paw volumes were measured and recorded at 0.5, 1, 2, 3, 4, 5, and 6 hours after carrageenan injection, respectively. Oedema indicated an increase in paw volume.
Fig. 1 Antinociceptive effects of aqueous (EA) and methanol (EM) extracts of the bulbils of Dioscorea bulbifera L. var sativa in pressure-caused pain. (Mbiantcha, 2011)
These results showed that the aqueous extract at 600 mg/kg exhibited anti-inflammatory activity that became significant after 2 h of carrageenan injection and remained at a maximum of 60.63% throughout the experiment. The methanol extract (300 and 600 mg/kg) induced a significant anti-inflammatory effect, which gradually increased and reached a maximum (80%) at the second hour. It showed that the aqueous and methanolic extracts of Dioscorea bulbifera L. var sativa have significant anti-injury perception and anti-inflammatory effects.
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Reference
- Mbiantcha, M.; et al. Analgesic and Anti-Inflammatory Properties of Extracts from the Bulbils of Dioscorea bulbifera L. var sativa(Dioscoreaceae) in Mice and Rats. Evidence-based Complementary & Alternative Medicine (eCAM). 2011, 8(1):1-9.