Tail Flick Test

Tail flick test is commonly used to measure the perception of injury to pain in rodents. The effectiveness of analgesics is evaluated by assessing the time it takes for animals to rapidly remove their tails from intense thermal stimulus.

Our company offers our clients a variety of tests based on thermal stimulation to test the sensitivity of animals to painful stimuli, including the classic tail flick test. We are committed to providing our clients with professional, personalized testing services in the evaluation of pain-related disorders or medications.

Introduction of The Tail Flick Test

Injury perception measures rely on physical indicators of discomfort, such as avoidance responses, licking, and vocalizations. In tail flicking test, the rodent's tail is stimulated using a high-intensity beam of light. In normal animals, the light beam produces a painful heat sensation that causes a reflection of the moving tail. In contrast, experimental animals subjected to genetic or pharmacological treatments delayed the response to pain from exposure to thermal stimuli.

Applications

Tail flick test is often used to study the neural basis of pain or the therapeutic potential of analgesic drugs. Adequate assessment of analgesic activity is important. The tail flick testing services we offered can facilitate our clients' progress in the following related researches.

• Basic research in pain caused by neurological diseases.
• Specific pharmacological studies of known analgesics.
• Development of new analgesics that exceed the effectiveness and safety of existing analgesics.

Example of The Application of The Tail Flick Test

The study described below evaluated the antinociceptive activity of the methanolic extract of the leaves of Feronia limonia Linn.

• Before administration, mice were immersed 2-3 cm at the end of their tails in hot water and kept at 55±0.5°C. The time for the appearance of the tail movement response was recorded and exceeded as 10 seconds.

Fig. 1 Tail flicking test evaluate the efficacy of analgesic drugs.

• The latency of pain response under different treatments was measured at 0, 30, 60 and 90 minutes after drug administration, respectively. The extract was administered to mice at three oral doses (100, 200 and 400 mg/kg body weight) with Diclofenac Sodium (25mg/kg) as standard.
• The time to pain response was significantly increased in mice given methanolic extract of F. limonia at 400 mg/kg body weight compared to control, and there was a dose effect on pain latency, indicating that methanolic extract of F. limonia has analgesic effect.

Fig. 2 Effect of methanolic leaves extract of F.limonia in tail flick test. (Saha, 2010)

Our company offers professional one-stop services from experimental design to results analysis to bring breakthroughs to your research. We guarantee on-time delivery of test results. If you need, you can contact us at any time.

Reference

1. Saha, M. R.; et al. Antinociceptive activity of methanolic extract of the leaves of Feronia limonia Linn. Bangladesh Journal of Physiology and Pharmacology. 2010, 26(1-2): 21-24.