he mitochondria play a critical role in ATP production, the primary energy currency of cells, through oxidative phosphorylation. This intricate process involves a series of redox reactions where electrons are transferred from electron donors to acceptors within the mitochondrial electron transport chain. As a result, an electrochemical gradient is established and utilized to drive ATP synthesis and generate the mitochondrial membrane potential (MMP). Protheragen provides specialized services that assess mitochondrial disease by offering assays capable of measuring MMP dynamics and enabling endpoint detection at specific time points.
Overview of the Mitochondrial Membrane Potential
Mitochondrial dysfunctions have been associated with mitochondrial disorders. The toxicity of exogenous compounds can disrupt various macromolecules in the mitochondria, leading to a reduction in mitochondrial membrane potential and subsequent effects on mitochondrial functions. Under normal physiological conditions, the mitochondrial membrane potential remains relatively stable. Prolonged disturbances can have detrimental effects on cellular health and contribute to the development of various pathophysiological conditions. Furthermore, a decrease in mitochondrial membrane potential may also be implicated in apoptosis. The dissipation of mitochondrial membrane potential has been utilized as an early indicator for apoptosis.
Fig.1 MMP assay principle. (Sakamuru, S., et al., 2023)
Our Services
The monitoring of mitochondrial membrane potential serves as a highly sensitive and early indicator for assessing mitochondrial integrity, making it indispensable in the identification of mitochondrial diseases and apoptosis. Leveraging fluorescent detection methods and diverse testing platforms, we are dedicated to delivering dependable and efficient services for mitochondrial membrane potential assays.
- Detection of dynamic changes in relative mitochondrial membrane potential.
- Endpoint detection of relative mitochondrial membrane potential at specific time points.
Our Technology Platform
Protheragen offers a dependable and efficient assay service for mitochondrial membrane potential, utilizing various testing platforms.
Cell Membrane-Permeable Fluorescent
Dyes
- 3, 3'-dihexyloxacarbocyanine iodide [DiOC6(3)]
- Rhodamine-123 (Rh123)
- Tetramethylrhodamine methyl and ethyl esters (TMRM and TMRE)
- JC-1
Detection Platform of Mitochondrial Membrane Potential
- Microplate reader
- Fluorescence microscopy
- Flow cytometry
- Single-Cell Electrophysiology Techniques
Acceptable Sample Type
The acceptable sample types for analysis of mitochondrial membrane potential encompass both adherent cells, which grow attached to a substrate, and suspension cells, which are freely suspended in a liquid medium.
Adherent Cells
Suspension Cells
Our Advantages
Protheragen offers a comprehensive mitochondrial membrane potential analysis service for mitochondrial disease, providing researchers with the necessary tools and expertise to unravel the intricacies of mitochondrial function and its implications in disease. Please feel free to contact us today for further information on how we can support your research endeavors and drive innovation in the field of mitochondrial biology.
Reference
- Sakamuru, S.; et al. (2022). Mitochondrial Membrane Potential Assay. Methods in molecular biology (Clifton, N.J.), 2474, 11–19.
