Mitochondrial Permeability Transition Pore Analysis

The mitochondrial permeability transition pore (mPTP) plays a crucial role in cell survival and apoptosis associated with mitochondrial disease. Leveraging expertise from experienced professionals and advanced techniques, Protheragen offers comprehensive services for assessing the activity of the mitochondrial permeability transition pore.

Overview of mPTP

The mitochondrial permeability transition (mPT) refers to a highly reproducible, Ca²⁺-dependent increase in the permeability of the inner mitochondrial membrane to ions and solutes. This phenomenon is mediated by a putative channel known as the mPTP, which has also been observed electro-physiologically and referred to as the mitochondrial mega-channel or multi-conductance channel. Activation of the mPTP can induce osmotic dysregulation, mitochondrial swelling, and cytochrome c release, initiating apoptotic cell death. Given that drug modulation can influence pore opening probability, it represents a valuable pharmacological target for translational studies in drug discovery.

Fig.1 Schematic representation of the current model of the mPTP. (Robichaux, D. J., et al., 2023)

Our Services

Our mPTP analysis service utilizes cutting-edge techniques and methodologies to dissect the intricate mechanisms underlying mitochondrial permeability transition pore (mPTP) function and dysregulation, enabling us to simultaneously assess both the integrity and potential of the mitochondrial membrane in cases of mitochondrial disease.

Workflow of Our Service

Following the workflow of our service, which encompasses the isolation and purification of mitochondria, as well as the assessment of their quality and function, the next crucial steps involve the preparation of assay buffer, addition of reagents, and precise measurement of pore opening.

Mitochondria Isolation and Purification

The initial step involves the isolation and purification of mitochondria from biological samples, typically cells or tissues. Mitochondria are separated from other cellular components using methods such as differential centrifugation or density gradient centrifugation.

Mitochondrial Quality and Function Assessment

Following isolation, the quality and functional integrity of the isolated mitochondria are evaluated. This assessment includes parameters such as mitochondrial membrane potential, respiratory activity, calcium handling capacity, and mitochondrial protein content.

Pore Opening Measurement

The assay begins with the addition of specific reagents or stimuli known to induce mPTP opening, such as calcium ions, oxidative stressors, or specific pharmacological agents. The response of the mitochondria to these inducers is then monitored in real-time or through endpoint measurements.

Data Analysis

Data obtained from the mPTP analysis are analyzed to quantify the extent of pore opening and assess mitochondrial responses to different stimuli.

Our Advantages

• Cutting-edge technology and advanced platform.
• Rich experience in detecting mitochondrial function analysis.
• Highly customized mitochondrial permeability transition pore assay.
• Rapid turnaround and cost-effective.

The Protheragen mitochondrial permeability transition pore analysis aims to enhance the comprehension of mitochondrial disease, thereby facilitating the development of innovative therapeutic strategies that target mitochondrial dysfunction. For inquiries or further information regarding our services, please do not hesitate to contact us.