The process of purifying mitochondrial proteins is an important step in exploring the functions of mitochondria and studying the diseases related to it. The first step to understand the complex changes of the protein profile, expression level, and modification level as well as to understand the function of the protein and its interactions is to isolate and purify mitochondrial proteins. Protheragen provides an integrated solution for isolating high-quality mitochondrial proteins from different specimen types.
Applications of Purified Mitochondrial Proteins
The purification of mitochondrial proteins has been widely utilized in the research field of mitochondrial disease.
- Functional Assays
The functional assays aimed at the mitochondrial bioenergetics, enzyme activities, different protein-protein interactions, and signaling pathways can be performed with purified mitochondrial proteins.
- Structural Studies
Purified mitochondrial proteins can be applied to X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and cryo-electron microscopy to determine three-dimensional structures and molecular mechanisms.
- Drug Discovery
Purified mitochondrial proteins are important in drug discovery and development, enabling screening for potential therapeutics for mitochondrial disorders, cancer, and other neurodegenerative diseases that stem from mitochondrial dysfunction.
- Biomarker Discovery
Purified mitochondrial proteins can be used to study and detect disease-specific mitochondrial biomarkers and other novel protein signatures of different diseases to aid in the diagnosis, prognosis, and therapy of different diseases.
Our Services
The purification process of mitochondrial proteins which is provided by Protheragen utilizes modern technology and stringent quality control measures. Each step, from sample preparation to purification, is done meticulously to ensure maximum yield and purity.
Sample Preparation
Sample preparation must be done in a way that the two parameters, namely integrity and yield of proteins are maximized. Depending on the sample type, this can be tissue homogenization, lysis of cells, or blood component separation.
Mitochondrial Protein Extraction
Extraction-appropriate detergents and buffers should be used to solubilize the mitochondrial membranes and extract the mitochondrial proteins while retaining their native structure and function.
Mitochondrial Isolation
Use differential centrifugation density gradient centrifugation, among other techniques, to isolate mitochondria from the cellular or tissue lysate. This step allows for the efficient isolation of mitochondria from other cellular components.
Protein Purification
Employ chromatographic techniques such as affinity chromatography or size exclusion chromatography to purify mitochondrial proteins based on their particular biochemical properties like size, charge, and affinity for particular ligands.
Our Advantages
- Expertise
Experienced scientists with expertise in the purification of mitochondrial proteins form our team.
- Customization
Purification processes are tailored to fit the needs of your particular research project.
- Quality
Supply of purified fractions of mitochondrial proteins with sufficient quality for assuring a range of downstream applications.
- Efficiency
Streamlined processes ensure rapid turnaround times without compromising on quality.
Regardless of whether you are working on the study of mitochondrial diseases, mitochondrial biology, or developing new therapies, we stand ready to assist you with our mitochondrial protein purification service to help achieve your research goals. Contact us today to discover how we can assist you in furthering your scientific work.
