Oxidative stress arises from an imbalance between the production of reactive oxygen species (ROS) and their removal by cellular antioxidant systems. Particularly in pathological conditions, mitochondria serve as a significant source of intracellular ROS generation. Furthermore, alterations in mitochondrial morphology are closely associated with changes in both intracellular ROS generation and mitochondrial function. Therefore, it is crucial to assess the normalcy of mitochondrial function through the detection of ROS changes. Protheragen employs various techniques to analyze and quantify changes in mitochondrial ROS levels.
The Importance of ROS Analysis
In essence, the analysis of ROS occupies a prominent position in scientific inquiry, providing a multifaceted approach to comprehending mechanisms and therapeutic development related to mitochondrial diseases.
- Disease Mechanisms
Our Mitochondrial ROS Analysis Service enables researchers to investigate the role of mitochondrial ROS in disease pathogenesis. By quantifying levels of reactive oxygen species (ROS) in disease models, researchers can elucidate the molecular mechanisms underlying mitochondrial diseases, providing insights into potential therapeutic targets.
- Drug Screening
Evaluating drug candidates for their ability to modulate mitochondrial ROS levels is crucial for drug development. Our service offers robust screening platforms to assess the efficacy of compounds in attenuating or enhancing mitochondrial ROS production. This facilitates the identification of novel therapeutics for mitochondrial diseases, including mitochondria-targeted antioxidants and ROS scavengers.
- Therapeutic Development
Understanding the impact of therapeutic interventions on mitochondrial ROS dynamics is essential for optimizing treatment strategies. Our service assists in evaluating the efficacy of drugs targeting ROS or modulating mitochondria in preclinical studies. Biomarker Discovery: Levels of mitochondrial ROS serve as potential biomarkers for disease diagnosis, prognosis, and treatment response.
- Biomarker Discovery
Levels of mitochondrial ROS serve as potential biomarkers for disease diagnosis, prognosis, and treatment response.
Our Services
The mitochondrial ROS analysis service we provide utilizes cutting-edge techniques and expertise to accurately quantify levels of ROS within mitochondria.
Fluorescent Probes
By utilizing fluorescent probes such as MitoSOX Red, MitoTracker, and 2',7'-Dichlorodihydrofluorescein diacetate (H
2DCFDA), we can selectively target mitochondria and detect levels of reactive oxygen species (ROS) through fluorescence microscopy or flow cytometry.
(EPR)
Electron Paramagnetic Resonance spectroscopy provides direct detection of ROS by measuring the paramagnetic properties of ROS-induced radicals. This technique allows for precise quantitative analysis of mitochondrial ROS production in both in vitro and in vivo experimental settings.
Biochemical Assays
Various biochemical assays, such as the quantification of lipid peroxidation products like malondialdehyde, protein carbonylation analysis, and DNA oxidation assessment, offer indirect measures of mitochondrial reactive oxygen species (ROS) levels.
Our Advantages
Welcome to inquire for further details. We will tailor a comprehensive service agreement based on your specific requirements and objectives. For additional cutting-edge technological services, please explore the remaining sections of our website or contact us.
