An imbalance characterized by the overproduction of reactive oxygen species (ROS) and the insufficient activity of cellular antioxidant systems leads to oxidative stress. Mitochondria are a major contributor to intracellular ROS, especially in the case of pathological conditions Additionally, the changes in the morphology of mitochondria are fundamentally linked to the intracellular levels of ROS and the activity of the mitochondria. Therefore, it is essential to evaluate mitochondrial function by measuring the rate of change in ROS levels. Protheragen employs a variety of methods to study and measure alterations in the levels of ROS produced by mitochondria.
The Importance of ROS Analysis
Primarily, the analysis of ROS is literally important for scientific research as it opens many avenues toward understanding mechanisms and providing therapy for those pertaining to diseases of the mitochondria.
- Disease Mechanisms
With our service on mitochondrial ROS analysis, the researcher becomes able to pursue the investigation of the role of ROS in mitochondrial disease pathology. Researchers can measure the level of reactive oxygen species in various disease models and then attempt to explain the molecular processes of mitochondrial diseases, providing insights into potential therapeutic targets.
- Drug Screening
It is necessary to assess drug candidates' capabilities of controlling mitochondrial levels of ROS before such drugs can be developed. Our services offer powerful screening systems to test how well compounds can stimulate or suppress mitochondrial production of ROS. Such strategies make it possible to treat those mitochondrial disorders with newly invented medications like antioxidants or ROS scavengers which have mitochondria as targeting sites.
- Therapeutic Development
Knowing how therapeutic modalities impact the levels of mitochondrial ROS is necessary for developing the best therapy regimens. Our service helps in assessing the effectiveness of drugs targeting ROS or manipulations of mitochondria in preclinical studies.
- Biomarker Discovery
The concentration of mitochondrial ROS makes good candidates for biomarkers for different disease diagnoses, their prognosis, and evaluating therapy effects.
Our Services
The mitochondrial ROS analysis service we provide utilizes cutting-edge techniques and expertise to accurately quantify levels of ROS within mitochondria.
Fluorescent Probes
Using fluorescent probes like MitoSOX Red or MitoTracker, as well as 2',7’-Dichlorodihydrofluorescein diacetate (H2DCFDA), permits the localization of mitochondria in addition to monitoring the production of ROS by fluorescence microscopy or flow cytometry.
Electron Paramagnetic Resonance (EPR)
Electron paramagnetic resonance spectroscopy is a technique that directly measures ROS by the detection of paramagnetic radicals produced from ROS. This method enables accurate measurements of mitochondrial ROS generation in vitro or in vivo experiments.
Biochemical Assays
Some biochemical indirect determination of mitochondrial reactive oxygen species levels includes quantification of lipid peroxidation products like malondialdehyde, protein carbonylation analysis, and DNA oxidation assessment.
Our Advantages
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