Genetically Engineered Animal Model Development

Genetically engineered animal models require intentionally modifying an organism's genetic makeup so that one or more genetic traits, or mutations, are incorporated. Protheragen with several specialists, provides services for developing genetically engineered animal models of cardiovascular diseases to assist scientists in studying the progression of diseases, testing novel therapies, and identifying potential drug candidates.

Overview of Genetically Engineered Animal Models

Genetic modifications of certain organisms enable scientists to unravel the underlying causes of various diseases and conditions. These models are crucial to scientific research. Using genetic engineering methods, scientists can add, delete, or change the genes with a known genetic anomaly of rare cardiovascular diseases to study the effects of these alterations in a controlled setting.

Fig.1 Modeling cardiovascular diseases using genome editing in mice. (Liu, N., and Olson, E. N., 2022)

Types of Genetically Engineered Animal Models for Rare Cardiovascular Diseases

Genetic engineering models have made it easier to understand the pathophysiology of rare cardiovascular diseases regarding genetic mutations. In addition, they serve as ideal platforms for assessing the efficacy of new therapeutic agents, determining dose-response curves, and forecasting possible side effects. Genetic engineering animal models for rare cardiovascular diseases are classified based on the method and strategy used to modify the genome, as well as the type of genetic alteration introduced. The following describes the main categories of genetic engineering animal models.

Knockout Models

These models involve the complete deletion or inactivation of a specific gene implicated in cardiovascular pathology.

Knock-in Models

These models targeted mutations, which very closely resemble the specific point mutations found in the individual, are inserted into the genome.

Transgenic Models

These involve the introduction and overexpression of exogenous genes (which are often in a mutated form) normally done under specific promoters.

Knockdown Models

The knockdown model utilizes techniques such as RNA interference (RNAi) or antisense oligonucleotides to reduce the expression levels.

Our Services

Building on our knowledge and skills in genetic engineering and cardiovascular disease modeling, we offer full-range services in the development of genetic engineering animal models to aid researchers in clarifying the intricate details of cardiovascular diseases and formulating novel therapeutic strategies.

Genetically Engineered Animal Models for Rare Cardiovascular Diseases

The table below lists some rare cardiovascular disease genetic engineering development services provided by our company. Should you wish to discuss any other rare cardiovascular disease animal model, please contact us and we will tailor a solution to meet your needs.

Genetically Engineered Animal Model Development Services

Animal Species Selection

• Mouse Model
• Rat Model
• Rabbit Model
• Zebrafish Model
• Pig Model
• Non-human Primates
• Others

Model Development Methods

• Knockout Model Development Service
• Knock-in Model Development Service
• Transgenic Model Development Service
• Knockdown Model Development Service

Disease Models

• Rare Arrhythmias
• Rare Cardiomyopathies
• Rare Congenital Heart Diseases
• Rare Vascular Diseases
• Rare Cardiac Tumors
• Other Cardiovascular Diseases

Workflow of Genetically Engineering Animal Model Development

Our Advantages

• Customized Model Development: Having significant knowledge and experience in modeling cardiovascular diseases, we construct and produce tailored animal models of cardiovascular conditions based on the researchers' needs.
• Advanced Technologies: Alter the genome of animals with high precision using advanced gene editing tools, enabling the production of precise disease models.
• Phenotyping Services: Provide reliable phenotyping services that incorporate physiological, molecular, and histological analysis of the animal models to ensure that research conclusions are accurate and appropriate.

Protheragen focuses on providing the best genetically modified animal models and offering solutions to meet the changing needs of your cardiovascular disease research projects, including pharmacokinetic studies and safety assessment services. If this piques your interest, do not hesitate to contact us so we can assist you in your research projects.

Reference

• Liu, Ning, and Eric N Olson. "CRISPR Modeling and Correction of Cardiovascular Disease." Circulation research 130.12 (2022): 1827-1850.