CAR-T Cell Preparation

Creating immune cells that are not only pure but also highly active is critical in cell therapy research and development. Being experienced in the area of immune cell preparation, Protheragen provides comprehensive CAR-T therapy development services, including autologous CAR-T cell and universal CAR-T cell preparation, ensuring efficiency and high-quality cell therapy development. Our scientists are quite adept at utilizing a range of methods to transfect CAR constructs into T cells, including viral transduction and non-viral transfection.

Overview of CAR-T Cell Preparation

Effective CAR-T cell preparation is needed for CAR-T therapies designed to treat hematologic cancers, solid tumors, and autoimmune diseases, as it determines CAR-T therapy safety, efficacy, and accessibility to individuals. There are some next-preparation approaches currently being developed to overcome specific constraints during CAR-T cell preparation.

Chimeric antigen receptors (CARs) are artificially designed proteins, the central role that each structural element within the CAR molecule plays in controlling T cell function. It is handily ascertained that numerous strategies focus on further improving the CAR design or combining its activity with other regulatory circuits to control functional aspects more precisely. There are non-viral methods like transfection and viral methods that employ vectors such as retrovirus or lentivirus, that can be utilized to transfer CAR genes into T cells.

Fig.1 The preparation process of autologous CAR T cells. (Abou-El-Enein, M., et al., 2021)

Gene Delivery Technologies

The transfection technique used to introduce the CAR transgene possesses great potential to alter CAR transgene expression and even cause genotoxicity, hence impacting the biosafety and biological potency of the developed CAR-T cell therapy.

Viral Gene Delivery

In trying to implement a CAR into T cells, gene editing viruses are prepared and introduced into the cells. The major viral vectors used comprise lentivirus, γ-retrovirus, and adeno-associated virus (AAV). It is reported that 94% of CAR-T products were created using viral vectors, over half of which were via lentiviral vectors.

Non-viral Gene Delivery

Additionally, non-viral transfection strategies for gene delivery have been developed for CAR engineering. In contrast to viral vectors, transposon systems, clustered regularly interspaced short palindromic repeats and their associated protein systems, and mRNA electroporation platforms are much more cost-effective.

Our Services

Advance the therapeutic of cancer and other illnesses by utilizing our top-notch CAR-T therapy development service. We have many methods for CAR-T gene transfection and provide services that encompass both regular and non-regular CAR-T cells. Furthermore, we offer fully tailored sets of CAR-T preparation services including T-cell isolation, genetic modification, and cell expansion based on our modern facilities and technology platforms.

CAR-T Cell Preparation Services

• By Technologies
  Viral Vectors: γ-retrovirus, Lentivirus, AAV, etc.
  Non-viral Vectors: Transposons System, mRNA Transfection, etc.
• By Types
  Autologous CAR-T Cell Preparation, Universal CAR-T (UCAR-T) Cell Preparation, Mouse CAR-T Cell Preparation, Others

As well as that, we offer tailored T cell immune phenotyping analysis relating to activation, exhaustion, and memory, also custom T cell analysis strategy creation, and multicolor flow cytometry test services aimed to offer you comprehensive and exact outcomes.

Based on the leading EndureCART^TM technology platform and comprehensive services, we cooperate with our customers during the entire CAR-T therapy development process including target identification to commercialization. Advancing the boundaries of cellular immunotherapy is possible with us as we have built up rich knowledge and resources, let's become partners. If you are interested in our technology platform and services, please contact us for more information.

For research use only, not for clinical use.