In Vitro Assay Services

In vitro analysis of CAR-T cells is necessary to assess the efficacy, target specificity, effectiveness, and safety which contributes to the further development of precision immunotherapy. Protheragen has extensive experience and an advanced technology platform in CAR T cell drug in vitro testing and efficacy assessment in vitro and can cater to your various evaluation needs. We can empower your research objectives and accelerate the development of next-generation CAR-T cell therapies with our in vitro assay services for CAR-T cells.

Overview of In Vitro Assay

In this process of CAR-T therapy development technology creation and CAR-T therapy enhancement, CAR-T cell in vitro assay has a powerful position. This assessment is based on experiments performed in the controlled environment of laboratories. Like other kinds of therapy, CAR-T cell therapy has to meet special characterization attributes about safety, purity, potency, identity, and stability in its evaluation.

Safety: Mycoplasma, sterility, endotoxin, quantification of residual viral agent, and viability.
Identity: Expression of CARs, visual appearance, dose, living cell content.

Potency: Antibody-mediated transduction efficacy, viability, CAR expression, toxicity, and cytokines (including IFN-y) secretion.
Stability: Composition, shipping, and storage.

Purity: T-cell purity, viability, quantification of residual reagents, transduction efficiency, and detection of any contaminating tumor cells.

Preparation of CAR T Cells. Fig.1 In vitro screens and assays of CAR-T therapy. (Kim, J., and Thomas, S. N., 2024)

In Vitro Assay Systems for CAR-T Therapy

Systems based on microfluidics are examples of in vitro systems that have acquired value as effective instruments to determine the quality and effectiveness of CAR T cell products. Such systems allow in-depth examination of CAR T cell properties, functions, and interactions in vitro and help in the estimation of how useful the therapies would be and improve the CAR T cell therapies.

Table 1 Microengineered in vitro systems for CAR T cell screens and assays

Systems	Assessment	Methods	Readout
Microfluidic	Cytotoxicity and cytokine levels	Co-cultivation with tumor cells in microwells	Granzyme B secretion and cytokine concentration
Microfluidic	Adhesion, migration, and cytotoxicity	Administration of CAR T cells and 3D co-cultivation with vascularized tumor-stromal spheroids	Fluorescence image
Well plate	Cytotoxicity	3D co-cultivation with organoids	Absorbance or fluorescence image
Microfluidic	Purity	Immunomagnetic separation of leukemic B cells	Flow cytometry

Our Services

At Protheragen, our scientists will comprehensively assess the post-transfection CAR-T cells and evaluate whether the construction of the CAR-T has been successful along with a functional verification. We customize and conduct a wide range of in vitro validation assays according to the requirements of your CAR-T cell engineering.

CAR Expression Analysis

For optimal results, it is suggested that determinations on the level of expression of the CAR gene be done at various stages of the CAR-T cell therapy process such as during post-CAR gene transfection, during the stage of cell preparation, and the period for therapy.

In Vitro Efficacy Evaluation

Evaluating the drug action in vitro is part of the CAR-T therapy development process and during this phase, the effector indicators of the cell therapy such as CAR-T usually include: CAR-T cell proliferation, CAR-T cell cytotoxicity, cytokine detection, etc.

Why Choose Us?

Extensive Experience
With a group of professionals is highly experienced specifically in CAR-T cell research as well as in vitro assays.

Advanced Technology
Equipped with advanced technologies and tools for conducting precise and reliable CAR-T cell in vitro assays.

Customized Approach
Each research project is different, so we customize our in vitro analysis services based on the demands of the clients.

Utilizing cutting-edge EndureCART^TM technology platform and advanced strategies to support CAR-T therapy development, our CAR-T cell performance assay services are attained through our in vitro models and innovative approaches which also inform us on how to enhance our therapeutic strategies for enhanced efficacy. If you are interested in our services, please do not hesitate to reach out to us at any time.

References

Si, Xiaohui et al. "Preclinical Evaluation of CAR T Cell Function: In Vitro and In Vivo Models." International journal of molecular sciences 23.6 (2022): 3154.
Kim, Jaehoon, and Susan Napier Thomas. "Microengineered in vitro CAR T cell screens and assays." Cell systems 15.12 (2024): 1209-1224.

For research use only, not for clinical use.

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Please note that we are not a pharmacy or clinic, so we are unable to see patients and do not offer diagnostic and therapeutic services for individuals.