Various pathogenic factors can result in structural and functional impairments within mitochondria. Precise detection and evaluation of mitochondrial damage are essential for the study and diagnosis of mitochondrial disorders. Protheragen offers an advanced technology platform for mitochondrial damage detection, enabling you to advance your project research.
Overview of Mitochondrial Damage
Mitochondrial damage is most often noticed as an increase in outer mitochondrial membrane permeability, which leads to the efflux of constituents from the intermembrane space into the cytoplasm, a decline in mitochondrial membrane potential, cleavage of inner mitochondrial membrane cardiolipin or revealing of the mitochondria 7A6 antigen. Mitochondrial disease is associated with damage to the mitochondria.
Applications of Mitochondrial Damage Detection Service
The applications of our mitochondrial damage detection service span a wide range of research areas.
- Drug Discovery and Development
Screening and validation of compounds targeting mitochondrial dysfunction for potential therapeutic interventions, including assessment of drug efficacy and toxicity in preclinical models.
- Biomarker Identification
Identification of novel biomarkers associated with mitochondrial damage for disease diagnosis, prognosis, and monitoring.
- Disease Mechanism Studies
Investigation of underlying mechanisms contributing to mitochondrial dysfunction in mitochondrial diseases.
Fig.1 Model for the mitochondrial damage for apoptosis induction by various proapoptotic compounds (Zorov, D. B., et al., 2014)
Our Services
Protheragen consistently offers tailored services for the detection of mitochondrial damage, and our comprehensive research platform with advanced analysis capabilities is designed to fulfill all your research requirements.
Molecular Analysis Service
- Next-generation sequencing (NGS) is used for the identification of mitochondrial DNA mutations and nuclear gene variants associated with mitochondrial disorders.
- Quantitative PCR (qPCR) is employed to measure gene expression levels related to mitochondrial function.
- Mitochondrial DNA copy number quantification is performed to assess mitochondrial biogenesis.
Biochemical Profiling Service
- Reactive oxygen species (ROS) detection assays to evaluate oxidative stress levels.
- Enzyme activity assays are conducted to evaluate the function of key mitochondrial enzymes involved in oxidative phosphorylation and metabolic pathways.
- Metabolomic profiling is carried out to characterize changes in metabolite levels associated with mitochondrial dysfunction.
Imaging Service
- Fluorescence microscopy for visualizing mitochondrial morphology and dynamics.
- Magnetic resonance imaging (MRI) and spectroscopy (MRS) are utilized for non-invasive assessment of mitochondrial function and tissue integrity.
- Positron emission tomography (PET) imaging with mitochondria-specific tracers allows visualization of in vivo mitochondrial activity.
Our Advantages
Protheragen aims to assist in research endeavors oriented towards solving mitochondrial disorders through comprehensive tests and specialized knowledge of our company's mitochondrial damage detection service. Please feel free to contact us for any additional services related to our extensive expertise and services.
Reference
- Zorov, D. B.; et al. (2014). Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release. Physiological reviews, 94(3), 909–950. https://doi.org/10.1152/physrev.00026.2013
