Adult Stem Cell (ASC)-derived organoids are epithelial structures with three-dimensional features that embody the specific attributes of the tissue from which they were formed. The organoids are appropriate model systems for examining Protheragen utilizes state-of-the-art adult stem cell technology to create 3D kidney organoids that accurately replicate the epithelial structure of the human renal tubules.
Introduction to ASC-Derived Kidney Organoids
With recent progress in stem cell research, it has become possible to produce ASCs derived kidney organoids. The ASC-derived kidney organoids have nephrons and other parallels of functional and distinctive attributes of the native kidney tissue. There are many advantages of using ASCs for therapies including lesser ethical objections and enabling patient-specific therapies from autologous cell sources. This is a significant step forward for applications in disease modelling, drug screening, and regenerative medicine in nephrology which is incredibly promising.
Fig.1 Kidney development in mammals. (Ibi and Nishinakamura, 2024)
Applications of ASC-Derived Kidney Organoids
Disease Modeling and Pathophysiological Studies
ASC-derived kidney organoids represent highly powerful in vitro models of human kidney development and disease for research purposes. These organoids allow those studying the molecular basis of kidney diseases to replicate the relevant features of human kidney structure and function.
Drug Screening and Nephrotoxicity Testing
One of the major difficulties for pharmaceutical companies is the prediction of the drug effect and toxicity on a human, living patient. The utilization of ASC-derived kidney organoids appears to be particularly useful for high-throughput drug screening and assessment of nephrotoxic potential. These organoids imitate the functions of filtration and reabsorption by the kidney.
ASC-Derived Kidney Organoids for Kidney Diseases
The focus of recent studies has been towards optimization of the cell source as well as the step-wise differentiation protocol to improve the maturation and functional capacity of the organelles into a kidney. Some classes of ASCs, including renal progenitor cells and urine-derived stem cells, have been investigated for their potential to undergo differentiation into many types of kidney cells.
Table1. Summary of cell sources, differentiation protocols, and terminally differentiated cells in ASC-derived kidney organoids. (Khoshdel-Rad, Ahmadi and Moghadasali, 2022)
| Cell source |
Differentiation protocol |
Cell-specific markers |
| Primary human renal epithelial cells |
R-spondin, EGF(50), FGF10 (100), Y (10), A8301 (5), primocin (0.1) |
Ezrin, OCT1, OCT2, ABCC1, ABCC3, ABCC4, SLC22A3, SLC40A1 (PT); PCBD1 (DT); CLDN10 and CLDN14 (Henle) |
| USCs |
Porcine K-ECM (14 days) |
NPHS1, SYNPO |
| Primary human renal cells |
KCM medium (30% FBS) and human K-ECM (14 days) |
NPHS1, SYNPO, PODOCIN |
| Primary mouse UBPCs |
LDN (200), TTNPB (0.1), C (3), JAKI (100), G (50), A83-01 (0.2), Rspo (110), FGF9 (50), SB (5) (10 days) |
PAX2, RET, GATA3, CDH1, KRT8, ETV5, WNT11 (UB stage), AQP2, AQP3, FOXI1 (CD stage) |
| Primary human UBPCs |
LDN (200), TTNPB (0.1 µM), C (3), JAKI (100), G (50), A83-01 (0.2 µM), Rspo (110), FGF7 (50), SB (5), Y (10), EGF (50) (10 days) |
KRT8, EMX2, LHX1, WNT11, RET, ETV5 (UB stage), AQP2, AQP3, FOXi1 (CD stage) |
Our Services
Protheragen provides complete services in developing ASC-derived kidney organoids to assist in research and drug development for kidney diseases. We create ASC-derived kidney organoids using stem cell technology for custom needs. This guarantees that the organoids keep their native features such as certain gene expressions, phenotypic features, and human kidney tissue specific molecular markers.
Workflow of ASC-Derived Kidney Organoid Development
Adult Stem Cell Isolation and Preparation
ASCs from the adult tissues of the specified donor organs are procured and pre-treated for organoid creation after quality control checks being the material of choice.
Directed Differentiation into Kidney Lineage
With the help of specialized techniques, Protheragen is able to influence ASCs to keratinocyte-specific lineages through certain patterns of growth factor and signal molecule application.
Organoid Formation and Maturation
The complex cells are placed in culture media which are two steps above the known media; they self-organize in three dimensions into kidney organoids and maturation occurs during the development of the nephron.
Comprehensive Characterization
In-depth quality control analysis of structures, molecules, and functions integrated to confirm the features of the organoids in order to guarantee the test quality standards expected.
ASC-Derived Organoid for Various Rare Kidney Diseases
With the known expertise, Protheragen offers numerous services providing the details in ASC-derived kidney organoid development which provide different platforms of focus for your research endeavors. Protheragen's deep understanding of
kidney disease modeling, makes it able to aid issues for all levels in ASC-derived kidney organoid development, bringing creative, flexible and direct answers that aid in your scientific objectives.
Why Choose Protheragen?
- Tailored Project Design: Protheragen understands that every research endeavor is unique, and therefore, offers flexible solutions tailored to specific client requirements for disease modelling, drug screening, or regenerative drug.
- Scalable Production Capabilities: Protheragen has the ability to adjust scale depending on project needs, whether it be small pilot studies or large-scale organoid production for high-throughput screening.
- Specialized Scientific Team: Protheragen employs a large group of scientists trained in stem cell biology, tissue engineering, and nephrology, whose extensive knowledge ensures that every project is done using the most up-to-date information and technology.
At Protheragen, we are dedicated to offering comprehensive, one-stop preclinical development services, specializing in ASC-derived kidney organoid development for kidney disease research. From
diseases model development to
drug safety evaluation, our services support every stage of your research process. If you are interested in our services, please don't hesitate to
contact us.
References
- Ibi, Y., and R. Nishinakamura. "Generating Kidney Organoids Based on Developmental Nephrology." Eur J Cell Biol 103.4 (2024): 151450.
- Khoshdel-Rad, N., A. Ahmadi, and R. Moghadasali. "Kidney Organoids: Current Knowledge and Future Directions." Cell Tissue Res 387.2 (2022): 207-24.
All of our services and products are intended for preclinical research use
only and cannot be used to diagnose, treat or manage patients.
