As a leading research services provider, we specialize in disease model development, delivering comprehensive and high-quality preclinical models to address the complexities of rare kidney diseases. Our services play a critical role in advancing the understanding of disease mechanisms and accelerating the development of targeted therapies.
Overview of Rare Kidney Disease Model Development
The development of rare kidney disease models is critical for understanding the complex pathophysiology of these conditions and advancing therapeutic research. Recent advancements, including the use of genetically engineered models, patient-derived xenografts, and organoid technologies, have enabled the creation of more precise and translationally relevant models. These innovations provide invaluable tools for studying disease mechanisms, identifying novel drug targets, and evaluating preclinical efficacy and safety.
Fig.1 Some of the most common disease models used in kidney research contribute to different aspects of our understanding of inherited kidney disease. (Molinari, E., and J. A. Sayer., 2020)
Types of Rare Skin Disease Models
Cell-based Models
These models use cultured renal cells to study disease mechanisms in a controlled environment. Techniques like CRISPR/Cas9 enable precise modeling of genetic kidney diseases, while patient-derived cells provide insights into specific conditions like IgA nephropathy.
Organoid Models
Kidney organoids replicate 3D microenvironments, closely mimicking human kidney structures. They are crucial for studying disease mechanisms, drug responses, and therapeutic strategies using advanced techniques like single-cell RNA sequencing and gene editing.
Animal Models
Genetically or chemically induced animal models, such as those for FSGS or AKI-to-CKD progression, provide systemic insights into kidney disease progression. These models are essential for evaluating the efficacy and safety of novel therapies.
Comparison of Kidney Disease Models
Different models are essential for studying kidney diseases, each offering unique insights and addressing specific research needs. From genetically modifiable mouse models to versatile 2D cell cultures and advanced 3D organoids, these systems vary in their complexity, relevance to human physiology, and experimental applications.
Table1. Summary of the specificities that distinguish the mouse, and two- and three-dimensional cell cultures as models in kidney research. (Molinari, E., and J. A. Sayer., 2020)
| Model |
Availability of Tools for Gene Manipulation in Reverse Genetics |
Suitability for High-Throughput Studies |
Similarity to the Human Genome |
Similarity to the Human Kidney |
| M. musculus (mouse) |
CRISPR/Cas technologies have significantly simplified the creation of knockout and knockin mouse models, which were previously achievable but complex using homologous recombination in embryonic stem cells. |
Due to high maintenance costs and a long reproductive cycle, mice are rarely used for large-scale genetic screens despite their utility in key studies. |
Mice and humans share approximately 70% of protein-coding genes |
The kidney's structure is similar in mice and humans, but humans have more nephrons due to differences in nephrogenesis regulation. |
| Two-dimensional cell culture |
Cell cultures are easily modified genetically, with RNAi and CRISPR/Cas9 being common tools for knockdown and knockout/knockin. |
Yes |
Depends on the species of origin. It is possible to obtain patient-specific cells |
N/A |
| Three-dimensional cell culture |
Kidney organoids and mIMCD-3 spheroids allow genetic studies, though core accessibility challenges transient modifications, while iPSC-derived organoids use CRISPR/Cas9 for PKD gene knockout. |
Yes |
Patient-specific URECs or iPSCs can be used to create kidney spheroids or organoids, depending on the species of origin. |
Spheroids: N/A
Kidney organoids are limited by reduced size, immaturity, poor vasculature, missing cell types, and lack of a branched collecting duct system. |
Disclaimer: Protheragen focuses on providing preclinical research services. This table is for information exchange purposes only. This table is not a treatment plan recommendation. For guidance on treatment options, please visit a regular hospital.
Our Services
At our company, we are committed to providing a comprehensive suite of services to support the development of disease models for rare kidney diseases. Our team of expert scientists leverages advanced technologies and extensive knowledge to create innovative and precise models tailored to your research needs.
Our Rare Skin Disease Model Development Services
Cell-based & Organoid Models
Development
- Primary Kidney Cell Line
- Immortalized Cell Line
- Renal Carcinoma Cell Line
- Kidney Progenitor Organoid
- Mature Kidney Organoid
Animal Models
Development Services
- Genetically Engineering Model
- Induced Disease Model
- Humanized Animal Model
- Syngeneic Model
- Xenograft Model
Types of Rare Kidney Diseases
Our organization offers expert services to address various rare kidney diseases, tailored to meet unique client needs. With extensive experience, we provide customized solutions to advance research and understanding of these complex conditions.
Why Choose Us?
Professional core
technical team.
Advanced experimental
equipment.
Empowering success
through cooperation.
Strict quality
control system.
With our comprehensive preclinical services portfolio, we support every stage of development, from target discovery through safety evaluation. If you are interested in our services, please don't hesitate to
contact us.
References
- Molinari, E., and J. A. Sayer. "Disease Modeling to Understand the Pathomechanisms of Human Genetic Kidney Disorders." Clin J Am Soc Nephrol 15.6 (2020): 855-72. Print.
- Tekguc, M., et al. "Kidney Organoids: A Pioneering Model for Kidney Diseases." Transl Res 250 (2022): 1-17.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
