The immortalized cell lines in cell biology and medical examination are of tremendous importance since they are cells that can be used reliably over and over again without losing the majority of qualities they had from the parent tissues. At Protheragen, we are focused on creating novel immortalized lines to support diagnostic and therapeutic innovation in rare kidney diseases.
Introduction to Immortalized Cell Line
An immortalized cell line is a population of cells that has undergone modification to multiply. This unlimited reproductive ability is the most defining characteristic of an immortalized cell line and is modified using typical genetic engineering. Immortalized cells provide a stable and homogeneous platform for experimental studies, in contrast to primary cells which have a limited life span and can show inconsistencies from one batch to another.
Fig.1 Engineering the HEK293 cell line for improved culture performance. (Abaandou, Quan and Shiloach, 2021)
Challenges in Immortalized Kidney Cell Line Development
Balancing Proliferation and Functional Integrity
Immortalization often disrupts renal cell-specific functions, such as podocyte slit diaphragm formation or tubular electrolyte transport. A study demonstrated that inducible immortalization systems (e.g., temperature-sensitive SV40) combined with differentiated media can transiently restore polarization in proximal tubule cells.
Preservation of Disease-Specific Signatures
Primary cells from rare kidney disease patients may lose genetic or epigenetic hallmarks during immortalization. CRISPR-Cas9-based epigenetic editing (e.g., maintaining PKD2 promoter methylation patterns) was shown in a paper to sustain cystogenesis potential in ADPKD models.
Immortalized Cell Line Development Strategies
- Techniques for Immortalization
With the aid of immortalised cell lines, scientists are able to model the mechanisms of rare kidney diseases in vitro by incorporating certain genetic variations, which are specific to the diseases, and cellular environments known to be associated with these diseases. The cell lines also enable scientists to study the mechanisms of disease development and progression at cellular and molecular levels.
- Disease-Specific Cell Lines
The possibility to generate immortalized cell lines from primary cells of individuals offers the best opportunity to retain the genotype and phenotype of the rare kidney diseases. This increases the fidelity of the in vitro models to the pathophysiological state of the disease for the purpose of studying its mechanisms and facilitating the design of therapies personalized to the diseased individual.
The development of co-culture systems incorporating immortalized renal cells with other cell types such as vascular endothelial cells enable researchers to investigate sophisticated cell-cell interactions that occur in the kidney. These systems aid in replicating tissue dynamics which are essential for kidney functioning, including understanding kidney physiology and discovering new therapeutic prospects.
Our Services
Protheragen actively works towards granting access to a wide variety of services for the development of immortalized cell lines to aid research on rare kidney diseases. The company employs a team of expert scientists and researchers with significant experience who use state-of-the-art genetic engineering tools to design dependable and precise immortalized cell lines, ensuring dependability at each and every level of the process.
Workflow of Immortalized Cell Line Development Service
Cell Selection and Culture
Immortalizing Gene Delivery
Clone Screening and Expansion
Functional Validation and Characterization
Immortalized Cell Line Development for Rare Kidney Diseases
Drawing on our vast knowledge, Protheragen offers an impressive variety of immortalized cell line development services aimed to fulfil the unique requirements of your study. Thanks to our extensive experience in kidney disease modeling, we can effectively tackle immortalized cell line development from every possible angle. We will provide advanced, precise, and personalized solutions to aid the achievement of your scientific objectives.
Why Choose Protheragen?
- Expertise in Techniques: Protheragen stands out by employing cutting-edge techniques for cell line immortalization, which is the creation of a cell line that retains its properties over time.
- Customized Genetic Engineering: Our skilled staff is able to perform target genetic modification of exceptional quality where specific mutations are both introduced and corrected in the immortalized cells.
- Comprehensive Support: We also specialize in the design of specific projects and provide a unique set of support services including client consultation prior to project start, assistance during the entire project preparation stages and post-delivery care.
- Client-Centric Collaboration: Flexible project scoping, transparent communication, and adaptable workflows to align with your research timelines and objectives.
Protheragen is dedicated to offering comprehensive, one-stop preclinical development services, specializing in immortalized cell line development for rare kidney disease research. From diseases model development to drug safety evaluation, our services support every stage of your research process. If you are interested in our services, please don't hesitate to contact us.
References
- Abaandou, L., D. Quan, and J. Shiloach. "Affecting Hek293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes." Cells 10.7 (2021).
- Reiter, S., et al. "Development of Immortalized Rhesus Macaque Kidney Cells Supporting Infection with a Panel of Viruses." PLoS One 18.5 (2023): e0284048.
All of our services and products are intended for preclinical research use
only and cannot be used to diagnose, treat or manage patients.
