Cell Line Development Service
Cell lines are cell cultures that are genetically modified to become immortal, and infinitely proliferative and passable. In the study of rare diseases, cell lines can be genetically engineered so that their properties meet the desired research for gene function study and drug screening, to circumvent the dilemma of scarce research resources. Our company provides professional services to construct stable cell lines according to your requirements.
Cell Lines for Rare Diseases
The immortalized cells have strong proliferation ability, can be passaged indefinitely, and can obtain a large number of cell resources in a shorter experimental cycle. Additionally, under the same genetic background, the stability of the experimental results can be guaranteed. A variety of cell immortalization methods have been established mainly based on the intervention of exogenous genes.
Commercial cell lines including HEK293T, Hela, K562 and NIH/3T3 have been used as models for various rare diseases. Through genetic engineering, the specific genes of the cell line is mutated or overexpressed to mimic the pathogenesis of the corresponding tissues. Additionally, immortalized cells derived from the same source as the diseased tissue provide a more suitable platform for the mechanistic study of rare diseases.
Fig. 1 Engineering the HEK293 cell line for improved culture performance (Abaandou et al. 2021) Potential Targets for Rare Diseases
A large number of rare diseases are caused by the loss of function of specific genes. Knockdown/knockout of specific genes through RNA interference or CRISPR/Cas9 technology leading to the loss of function is one of the essential strategies to study the role of these genes in various biological processes of rare diseases. A typical example is the inactivated mutations in DNA methylation-related genes affecting the epigenome of cells, consequently attributing to some rare diseases.
Table 2 DNA methylation-related genes known to cause rare diseases (Martinez-Delgado and Barrero 2022)
| Function | Gene Symbol | Disease |
|---|---|---|
| DNMT | DNMT1 | Cerebellar ataxia, deafness, narcolepsy, autosomal dominant |
| Neuropathy, hereditary sensory, type IE | ||
| DNMT3A | Heyn–Sproul–Jackson syndrome | |
| Tatton–Brown–Rahman syndrome | ||
| DNMT3B | Facioscapulohumeral muscular dystrophy 4, digenic | |
| Immunodeficiency–centromeric instability–facial anomalies syndrome 1 | ||
| MBDcontaining protein | MECP2 | Rett syndrome |
| MBD5 | Intellectual developmental disorder, autosomal dominant 1 | |
| GATAD2B | GAND syndrome |
Our Services
Cell lines are routine experimental models for preclinical research. Our company provides services of constructing stable cell lines with transparent genetic background and no contamination of miscellaneous cells, providing a convenient strategy for analyzing the pathogenesis and drug response mechanism of rare diseases. Our services include but are not limited to:
Cell Immortalization Service
The immortalization protocol is designed according to the client's cell type to select the most appropriate and efficient viral or human immortalized gene for insertion.
Knockdown / Knockout Cell Line Service
The gene knockdown / knockout programs are selected according to the idea of clients, and obtain monoclone with gene inactivated function by strict screening and identification standards.
Fluorescent Cell Line Service
The overexpression system is adapted according to the purpose of clients to establish cell lines stably expressing target genes and fluorescent proteins through efficient screening methods.
Starting with the DNA of your gene of interest, through to obtaining a stable cell line that meets your needs, we provide you with an experienced team and necessary resources to support your cell line research in rare diseases as the following workflow:
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Vector Construction
- DNA Synthesis
- DNA Amplification
- Fusion with fluorescent protein
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Transfection
- Chemotransfection
- Electroporation
- Vira-vector Method
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Selection
- Stable Clone Pools
- Limiting Dilution
- Antibiotics Selection
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Validation
- Productivity Measure
- Expression Confirmation
- Functional Validation
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Delivery
- Stability Testing
- Clone Generation Report
- COA Reports
Our Advantages
Precise gene editing with short cycle time
Rigorous and advanced cell line development platform
Free of contamination, high viability and proliferation
Comprehensive certificate handling procedure services
Integrated with extensive project experience and professional researchers in the field of molecular biology and cell biology, our company offers you with diverse cell line development services to assist you to study the unresolved mechanisms of rare diseases, to screen the promising drug targets and to test new treatment modalities. We are committed to the quality of the establishment of cell lines and provide a full range of services and materials for your research. If you are interested in our cell line development service, please contact us for more information.
References
- Abaandou L, et al. Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes. Cells, 2021, 10 (7).
- Martinez-Delgado B, et al. Epigenomic Approaches for the Diagnosis of Rare Diseases. Epigenomes, 2022, 6 (3):21.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.