Baculovirus Vector Development Service
Recombinant baculoviruses can deliver therapeutic genes to target cells by ex vivo or in vivo methods and are expected to be good gene therapy vectors. Our company is committed to helping customers develop a variety of cutting-edge modified baculovirus vectors to improve the efficiency of therapeutic gene expression in vivo, which is key to the success of therapeutic development for multiple rare diseases.
Background
Gene therapy can be adapted to each individual to treat a wide range of diseases including cancer and rare diseases. The development of suitable gene transfer vectors to introduce exogenous genes into target cells is of great importance. Currently, research in gene therapy vectors has developed from viral vectors such as retroviruses, adenoviruses, and adeno-associated viruses to today's novel vectors such as baculovirus vectors, which provide a relatively safe, scalable, and cost-effective gene transfer strategy for gene therapy.
The baculovirus gene delivery system is an easily modifiable gene therapy system that has been reported to enable site-specific delivery, mitigate adverse effects and improve therapeutics, with the potential to be the cost-effective and efficient backbone for gene therapy. Although challenges remain for recombinant baculoviruses in gene therapy, their high DNA-carrying capacity, maneuverability, and ability to express multiple exogenous genes simultaneously make them promising as gene delivery systems for personalized gene therapy.
Fig. 1 Putative model of internalization of baculovirus into mammalian cells. (Ono C, et al., 2018)
Our Services
Based on our extensive knowledge and advanced technology in structural biology, biochemistry, and cell biology, we provide our customers with safe and effective baculovirus vectors for gene delivery, which can help accelerate the development of gene therapies for a number of rare diseases such as Duchenne muscular dystrophy (DMD). Our services include, but are not limited to:
- Genetic modification of baculovirus for minimum vectors
We provide genetic modification services for baculoviruses by reducing their genome size. In order to optimize baculovirus expression vector system (BEVS) with the minimum baculovirus genome, we provide a variety of strategies, such as gene deletions or replacing some loci in the baculovirus genome with foreign gene expression cassettes. - Development of gene delivery systems based on baculoviruses
We help customers improve the efficiency of transduction and simplify production by modifying and manipulating the baculovirus genome. We have developed several vector systems such as BacMam and Bac-to-Bac systems to provide the bases for site-specific gene delivery. - Production of baculovirus for gene therapy
We provide several simple and effective modifications to significantly enhance the feasibility and efficiency of baculovirus production for gene therapy as well as improve gene transcription and delivery, such as changing the chromatin state and media supplements. - Improvement of the efficiency of gene delivery
The ability of virus vectors to enter the target cell type is affected by cell type, chromatin status, and promoter type. We provide the following strategies to help customers improve gene delivery efficiency: - Baculovirus promoter selection for mammalian cell entry in rare disease gene therapy. We provide multiple combinations of viral and mammalian promoters to achieve adaptability and customization in baculovirus gene delivery.
- Modification of baculovirus surface to enhance transduction efficiency, involving the addition of several molecules such as vesicular stomatitis virus G (VSVG) and single-chain antibody fragments to the surface of baculoviruses.
- Prolonging baculovirus transgene expression for rare disease gene therapy by methods such as hindering complement activation.
- Purification of baculovirus vectors for rare disease gene therapy
We have established efficient purification and detection procedures to provide customers with high titers of baculovirus vectors free of incidental contaminants and endogenous viruses.
Applications
- Development of baculovirus-based vaccination vectors
- Development of baculovirus vectors for cancer gene therapy
- Development of baculovirus vectors for tissue engineering
Our company has unparalleled knowledge and best-in-class technology in the development of gene delivery tools. Our specialist one-stop baculovirus vector development service helps to address the low capacity, the need for replication helper viruses, and the biosafety issues of existing gene therapy vectors. If you are interested in our services, please feel free to contact us for more details.
Reference
- Ono, C.; et al. Baculovirus as a tool for gene delivery and gene therapy. Viruses, 2018, 10(9): 510.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.