Liposome Development Service
Liposomes are considered to be an ideal drug carrier system, capable of binding a wide range of substances. Our company is committed to providing customers with professional and reliable services for the development of liposomes for gene delivery, including the preparation, production, and characterization of liposomes. Our excellent technology platform ensures high quality and effectiveness in all aspects of liposome development, accelerating the discovery of gene therapies for rare diseases.
Background
Liposomes are bilayers of lipids arranged in an orderly fashion to form closed vesicles, also known as artificial biofilms. As lipid-based drug carriers, liposomes offer unique advantages including good biocompatibility, morphological and compositional diversity, lack of immunogenic response, the ability to encapsulate and protect a wide range of therapeutic biomolecules, and differential release properties.
Currently, liposomes are widely studied and used as potential drug carriers in the therapeutics of major diseases, offering significant promise for the development of gene therapies for rare diseases. For example, novel cationic liposomes have been developed as a non-viral gene delivery vector for the therapeutics of Lafora disease, which is neurodegenerative epilepsy. In addition, liposomes open up new opportunities for the therapeutics of lysosomal storage disorders (LSD), and gene therapy using liposomes is being investigated for the development of effective therapeutics for LSD.
Fig. 1 Schematic representation of the different generations of liposomes. (Tomsen-Melero J, et al., 2022)
Our Services
We offer a wide range of liposome engineering services as well as customized services to produce liposomes of the required size, volume, and laminarity. Our researchers have developed multiple strategies to help customers effectively modify liposomes to obtain gene delivery systems with the benefits of target-specific delivery, enhanced intracellular penetration, etc. Specifically, we ensure safe and effective gene delivery by modulating parameters related to physicochemical properties, including solubility, degradability, molecular weight, charge density, crystallinity, stiffness, hydrophobicity, and pKa values of cationic nanocarriers. We offer the following services to support your research.
- Preparation of liposomes
We have established various protocols to help customers prepare liposomes with desired qualities. Our methods include the thin-film hydration method, Bangham method, reverse-phase evaporation, solvent dispersion method, ethanol injection, microfluidic method, dual asymmetric centrifugation (DAC), supercritical reverse-phase evaporation method (SRPE), spray drying, membrane contactor technology, and cross-flow injection technique. We choose the most appropriate preparation method according to the needs of our customers. - Optimization and development of liposomes
We provide a variety of optimization strategies to improve the customer's success in developing appropriate gene delivery vectors for gene therapy of rare diseases. Our strategies include but are not limited to:- We help customers develop long-circulating liposomes by surface functionalizing traditional liposomes with inert polymer molecules (such as glycoproteins, oligosaccharides, and polysaccharides).
- We help our customers develop ligand-targeted liposomes to ensure the active targeting of nucleic acids to target tissues through the conjugation of molecules that bind to tumor tissue receptors with therapeutic nucleic acid-loaded liposomes.
- We enhanced the transfection efficiency of cationic liposomes by incorporating a helper lipid such as 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) or cholesterol.
- Characterization of liposomes
We provide various characterization techniques, such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic size exclusion chromatography (SEC), Zeta potential analysis, and Cryo-TEM technique to help customers study the features of liposomes.- Analysis of size and size distribution
- Analysis of surface charge and stability
- Evaluation of the lamellarity of liposomes
- Analysis of encapsulation efficiency
Our company has been dedicated to the research and development of liposome technology for many years and has been a trusted provider of liposome services for customers globally. We offer a wide range of liposome-based vectors and engineering services to meet your diverse needs in the field of gene therapy and we promise to provide you with the best products and first-class service with our advanced technology. Please contact us for more details and to get a formal quote.
Reference
- Tomsen-Melero, J.; et al. Liposomal formulations for treating lysosomal storage disorders. Advanced drug delivery reviews. 2022: 114531.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.