Naked DNA Development Service
As a top-ranking service provider in the field of gene therapy, our company focuses on developing the latest technologies to obtain efficient and safe non-viral gene delivery systems. Equipped with a superior platform and state-of-the-art equipment, we offer high-quality and efficient naked DNA development and customization services to help you accelerate progress in gene therapy for rare diseases.
Background
Naked DNA is the simplest non-viral vector that can be injected directly into specific tissues, particularly muscle, and can achieve high levels of gene expression. Naked plasmid gene therapy drugs, which are drugs developed using naked plasmids as the target gene vector, have become a more common form of gene therapy. The number of gene therapy research using naked plasmid DNA as a vector is now roughly comparable to those using adenovirus and retrovirus vectors worldwide. Compared to viral vectors, naked plasmid vectors have the advantage of lower immunogenicity and toxicity, do not carry the risk of gene integration, and are easier to produce, transport and store, making them more conducive to mass production.
The simplicity of naked DNA-based gene transfer has generated great interest in its use in research for gene therapy. Currently, the majority of naked plasmid gene therapy drugs are in the early stages of research for a variety of diseases, including cancer, heart disease, peripheral neuropathy, and skeletal disorders. Given the inherent simplicity and ease of production of naked DNA, it may be an effective option for future gene therapy for rare diseases.
Fig. 1 The differences in the cellular effect of viral vs. naked DNA vectors. (Melman A, et al., 2009)
Our Services
Our researchers provide our customers with specialist scientific services and solutions to address the challenges in the field of naked DNA-mediated gene therapy. Our services include, but are not limited to:
- Synthesis of naked DNA
We use recombinant DNA technology to help our customers synthesize naked DNA.- PCR amplification. Our process involves PCR amplification of the desired DNA and purification and quantification of the region of interest, with direct injection using available vectors.
- Gene cloning. We use restriction digestion or physical methods to integrate the extracted DNA into the plasmid. The gene copies are then isolated, purified and used for transfer.
- Delivery of naked DNA into cells
Plasmid DNA, as a negatively charged polymer with a large molecular weight, is unable to cross the cell membrane freely and enter the cell. We offer a variety of strategies to facilitate the entry of DNA into cells.- We increase the efficiency of naked DNA uptake by adding additional sequences such as polyadenylated DNA, promoters, antibiotic resistance genes, and protein-specific ORFs.
- We use synthetic capsules such as liposomes to efficiently transfer naked DNA into the host cell, which protects naked DNA from nuclease attacks.
- We offer techniques such as electroporation, hydrodynamic-based methods, gene guns or particle bombardment to inject DNA directly into cells or tissues.
- We also offer other techniques such as photoporation, sonication, hydroboration, and magnetofection to increase the chances of DNA intake.
- Performance analyses of minicircle vectors
We provide the evaluation of the transfer and expression efficiency of minicircle-based vectors in vitro and in vivo by flow cytometry analysis (FACS), fluorescence microscopy, qRT-PCR, and other techniques.
Why Choose Us?
- Extensive experience in naked DNA for gene delivery
- Fast turnaround time and strict adherence to schedules
- Custom service
- Ensure that every step is well organized with satisfaction guaranteed
- Reasonable prices and excellent service
As a leading biotechnology company in the field of delivery systems, we have the strength to provide high-quality naked DNA services for customers. Our research team of professional experts with many years of experience offers free consultation and project feasibility analysis of naked DNA as gene delivery vectors, and ultimately develops the best solution for you. For more information, please feel free to contact us.
Reference
- Melman, A.; Davies, K. P. Gene therapy in the management of erectile dysfunction (ED): past, present, and future. The Scientific World JOURNAL, 2009, 9: 846-854.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.