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Knock-in Model Development Service

Within the context of development of drug and therapies for a rare disease, the knock-in models stand out as being unique because they enable researchers to decode the puzzles of a genetic disorder and understand the disease phenomenon more thoroughly. Our company having years in the industry, offers specialized services in the development of knock-in models. Guided by a commitment for scientific achievement through innovation and collaborative partnerships, we work towards faster invention and development of new therapeutics for a spectrum of rare diseases.

Introduction to Knock-in Models

Knock-in models are genetically altered organisms, either a mouse or some other species, that have had a gene of interest added or substituted to at least one of its other existing genes which can simulate mutation of diseases in children. Models are at the forefront of genetic studies and as such brand themselves into the depths of perplexity and burstiness of molecular intricacies. These models, which include reporter mice with integrated genes for human SNPs, highlight the possibilities of further genetic modifications adding human complexity.

Just as a cosmic tracker sets out to explore uncharted regions, scientists use knock-in mice to recreate human diseases, verify the effectiveness of certain compounds, explore the mysteries surrounding the activity of genes that act as promoters, identify the hidden functions of mutated proteins, map out the complicated routes of cellular movement, conduct gene replacements with reporter genes, and undertake audacious projects aimed at humanization.

Strategies of Knock-in Model Development

Knock-in models utilize different methods to genetically engineer animals and simulate disease-related mutations. The methods enhance comprehension of the gene's purpose and the effect of the germline mutation on the pathological processes of the illness. The table below provides a comparison of three new and innovative approaches designed for the knock-in model:

Table 1 Comparison of knock-in strategies. (Miura, Hiromi, et al., 2018)

Our Services

As a leading company in the field of biological research, our commitment to advancing knock-in models is unwavering. We employ cutting-edge gene-editing technologies to create reliable knock-in models, and used the models for drug safety evaluation and pharmacokinetics studies. Our team of experienced scientists works tirelessly to collaborate with researchers globally, offering tailored solutions to meet their specific research needs.

Our company, with its commitment to pioneering the field of biological research, continues to progress with the development of knock-in models. Our experience enables us to develop precise knock-in models with today's most advanced gene-editing technologies, which we have applied for drug safety evaluation and pharmacokinetics studies. A seasoned workforce of scientists in our company works constantly and in collaboration with researchers across the world in order to provide specific solutions to different research problems.

• Animal Species for Model Development

• Methods of Knock-in Model Development

• Types of Knock-in Model Development
  Constitutive / Conventional / Whole-body Knock-in Models:
  A point mutation technique allows the homology arms or a targeting vector to be changed resulting in the appropriate mutation being integrated in the PCR amplified gene of interest. Such mutations are expected to be stably expressed under the regulatory control of the wildtype gene.
  Reporter Knock-in Models:
  A reporter gene is placed at the 5' or 3' end of the coding region, yielding a fusion protein while enabling the monitoring of gene expression by focusing on transcription or translation, as well as examining the movement and positioning of proteins within cells.
  Quick (Rosa26 and Hprt) Knock-in Models:
  We provide mouse and rat knock-in models, where transgenes are integrated at predefined safe HPRT and Rosa26 loci.
  Humanized Knock-in Models:
  Humanized genetically engineered mouse models are created by replacing the mouse endogenous gene with its corresponding human ortholog.
  Point Nutation Models:
  Knock-in of the target gene is achieved by constructing point mutation models.

Why Choose Us?

With more than a decade of experience in researching and developing therapies for rare diseases, our company provides complete services to established and growing biopharmaceutical companies and even scientific research centers. We focus in the development of knock-in models for rare diseases using our proprietary platform. We are proud to state that we offer superior services and products tailored to suit the specialized needs of our clients. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

• Miura, Hiromi, et al. "Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors." Nature protocols 13.1 (2018): 195-215.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

• Knock-out Model Development Service
• Knock-down Model Development Service
• Point Mutation Model Development Service
• Overexpression Model Development Service