Overexpression Model Development Service

Manipulation of gene expression has made great strides in helping address rare genetic disorders. It aids in determining the function of genes, studying their pathways, and finding possible treatment methods. In this regard, overexpression models are becoming highly useful by allowing gaging of the consequences of gene overexpression in multiple biological systems. Our company is the first to provide complete comprehensive services of overexpression model development as a means to facilitate drug discovery for orphan diseases.

What is an Overexpression Model?

An overexpression model is a genetically modified organism, typically a plant or an animal, in which a specific gene or genes are artificially amplified to achieve higher expression levels than what is observed in the normal state. This manipulation allows researchers to study the effects of gene overexpression in a controlled setting. Overexpression models provide valuable insights into gene function, molecular pathways, and the impact of gene overexpression on biological processes.

Fig.1 Schematic representation of CCNE1 overexpression. (Aziz, Khaled, et al., 2019)

Transgenic technology is the process of integrating genes from one organism into another. This process enables scientists to focus on the enhancement of a particular gene's expression. The use of certain promoters and enhancers can greatly increase the level of expression to the point of overexpression in the specific targeted tissues or cells.

Tools of Overexpression Model Development

The tools of explore transgene expression and overexpression cDNA and open reading frames (ORF) construct are important tools that stem from the native mRNA template and greatly aid the expression as well as overexpression studies.

Our Services

The use of overexpression models in studying the functions of genes, the pathophysiology of rare diseases, and creating novel therapies is groundbreaking. As one of the most developed companies in the field of genetics research and services, we provide contractors with complete overexpression model development services including drug safety evaluation and pharmacokinetics studies. With the use of a transgenic system and any type of viral vectors for gene editing, we are capable of developing customized overexpression models for the client's specific research objectives.

• Animal Species for Model Development
  -Mouse
  -Rat
  -Rabbit

  -Pig
  -Dog

  -Monkey
  -Cat

  -Zebrafish
  -Others
• Types of Overexpression Model Development
  • Rosa26-based Overexpression Models
    One of the most commonly used site-directed integration sites, Rosa26, is used to construct an overexpression animal model by inserting exogenous DNA in this region.

    Our company can perform site-specific integration of very large fragments (20-30kb). There are also multiple sites available to meet different research needs, including Col1a1, AAVS1, H11, CCR5, HPRT, TIGRE.
  • PiggyBac (PB) Transposon-based Overexpression Models
    The target fragment is cloned into the PiggyBAC transposon plasmid and injected into mouse-fertilized eggs together with the transposase. Transgenic mice are obtained by integrating the target fragment with the TTAA site on the genome.

    The luciferase reporter gene is used to quickly determine the gene expression of founder mice through an in vivo imaging system.

Why Choose Us?

Models of overexpression serve various purposes, such as in functional genomics, identification of drug targets, and mechanistic analysis of rare disorders. Utilizing our know-how in overexpression model development, phenotypic analysis, and data interpretation, our company is fully equipped to assist scientists in pursuing their research goals using overexpression models. A group of experienced scientists and genetic engineers carry out and integrate modern technologies and approaches to provide the best results. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

• Aziz, Khaled, et al. "Ccne1 overexpression causes chromosome instability in liver cells and liver tumor development in mice." Gastroenterology 157.1 (2019): 210-226.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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