Overexpression Model Development Service

In the rapidly evolving landscape of rare diseases, the ability to manipulate gene expression plays a crucial role in unraveling gene function, deciphering disease mechanisms, and exploring potential therapeutic interventions. Overexpression models have emerged as powerful tools within this field, enabling researchers to gain valuable insights into the effects of gene overexpression across different biological systems. Our company is at the forefront of offering comprehensive overexpression model development services. These services are designed to expedite the discovery of novel drugs for rare diseases.

What is an Overexpression Model?

An overexpression model is a genetically modified organism, typically a plant or an animal, in which a specific gene or genes are artificially amplified to achieve higher expression levels than what is observed in the normal state. This manipulation allows researchers to study the effects of gene overexpression in a controlled setting. Overexpression models provide valuable insights into gene function, molecular pathways, and the impact of gene overexpression on biological processes.

Fig.1 Schematic representation of CCNE1 overexpression. (Aziz, Khaled, et al., 2019)

Transgenic technology involves the introduction of foreign genes into the genome of an organism. This technique allows researchers to specifically target and amplify the expression of genes of interest. By using appropriate promoters and enhancers, gene expression can be significantly increased, leading to overexpression in the desired tissues or cells.

Tools of Overexpression Model Development

The tools of complementary DNA (cDNA) and open reading frames (ORF) are derived from native mRNA, serving as valuable aids in gene expression and overexpression assays.

Our Services

Overexpression models have revolutionized the exploration of gene function, disease mechanisms, and therapeutic interventions in the field of rare diseases. Our company, a leader in genetic research and services, offers comprehensive overexpression model development services, including drug safety evaluation and pharmacokinetics studies. By leveraging advanced tools and techniques, such as transgenic technology and viral vectors, we can precisely manipulate gene expression and generate customized overexpression models tailored to specific research needs.

• Animal Species for Model Development
  -Mouse
  -Rat
  -Rabbit

  -Pig
  -Dog

  -Monkey
  -Cat

  -Zebrafish
  -Others
• Types of Overexpression Model Development
  • Rosa26-based Overexpression Models
    One of the most commonly used site-directed integration sites, Rosa26, is used to construct an overexpression animal model by inserting exogenous DNA in this region.

    Our company can perform site-specific integration of very large fragments (20-30kb). There are also multiple sites available to meet different research needs, including Col1a1, AAVS1, H11, CCR5, HPRT, TIGRE.
  • PiggyBac (PB) Transposon-based Overexpression Models
    The target fragment is cloned into the PiggyBAC transposon plasmid and injected into mouse-fertilized eggs together with the transposase. Transgenic mice are obtained by integrating the target fragment with the TTAA site on the genome.

    The luciferase reporter gene is used to quickly determine the gene expression of founder mice through an in vivo imaging system.

Why Choose Us?

Overexpression models have diverse applications in functional genomics, drug target identification, and mechanistic studies of rare diseases. With our expertise in overexpression model design, phenotypic characterization, and data analysis, our company offers a comprehensive suite of services to support overexpression model development and advance scientific research. Our team of experienced scientists and genetic engineers utilizes state-of-the-art technologies and methodologies to deliver high-quality results. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

• Aziz, Khaled, et al. "Ccne1 overexpression causes chromosome instability in liver cells and liver tumor development in mice." Gastroenterology 157.1 (2019): 210-226.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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