Protein Detection Service
It is essential to comprehend the composition, structure, and function of a protein as it relates to the quality and effectiveness of drugs for a particular disease. Therefore, our enterprise offers a range of services in the detection of proteins which aid in elucidating the protein composition, structure, and function of biological drugs. We can provide you with the key services in the development of drugs and optimization of therapy schemes for orphan diseases.
Introduction to Protein Detection
The detection of protein composition, structure, and interaction is the first step in the drug development of orphan disease proteins. Routine methods for protein detection are categorized into two classes: immunology and mass spectrometry. Immunology involves the specific binding of antibodies with their respective proteins. The common ones are ELISA and Western blot that screen for the presence of the specified protein. Mass spectrometry is utilized for the protein quantification in a given sample by determining the mass and relative quantities of protein molecules. It is used to screen proteins with post-translational modifications, interacting proteins, and proteins in complexes.
Fig.1 Overview of mass spectrometry (Neagu, Anca-Narcisa, et al. 2022)
Application of Protein Detection in Rare Diseases
- Early diagnosis of rare diseases
There are new types of tumors, uncommon heart issues, and unusual nervous systems disorders, as well as tumors having corresponding organs within organisms that overtly showcase the abnormal expression of certain proteins within them that reflect towards the existence and development utilizing protein detection technology. - Accurate diagnosis of rare diseases
Protein research methodology attainment gives organisms precise and thorough information regarding the proteins within them enabling accurate disease identification by analyzing the presence and expression levels of the proteins in question. - Targeted therapy for rare diseases
Undescribed medical illnesses undergo specific therapy and diagnosis through the advancement of technology that allows the study of differences in proteins using of merit. Targeted and single cures for ailments are utilized through personalized medicine.
Our Services
Our services in protein detection and analysis allow specialists to investigate dosage form interactions with certain proteins and as such the mechanism of action of the specific drug. Our company has extensive experience in providing protein analysis and detection services and as such we can offer the following selection of protein detection services:
Western Blot
- Medium-sized target proteins as low as 1 to 5ng can be detected
- Identify the expression of the target protein
- Mature technology and reliable results
ELISA
- Detect the quantitative and qualitative expression of peptides, antibodies, and hormones
- Mature technology and low-cost
- High specificity
IP and Co-IP
- Study the rational interaction of two proteins in cell endogenesis
- Identify a new partner for a particular protein
GST pull-down
- Study the interaction between two proteins
- Verify the binding of the two proteins in vitro
- Simple method and operation easy
Mass spectrometry
- Identification of protein modification sites
- Identification of protein after dye
- Identification of unknown proteins in complex samples
Edman degradation
- Protein DeNovo sequencing
- Protein N-terminal sequencing
Project Workflow
Our company has the capabilities and resources to provide professional communication and problem-solving support to ensure that we can quickly respond to the changing needs of your rare disease therapy research projects. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.
Reference
- Neagu, Anca-Narcisa, et al. "Applications of tandem mass spectrometry (MS/MS) in protein analysis for biomedical research." Molecules 27.8 (2022): 2411.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.