Protein Expression and Purification Service

Proteins with specific functions can be produced through protein expression and purification services, which play an important role in the therapies of rare diseases. Our company has a variety of different recombinant protein expression systems, which can provide services from gene synthesis and carrier construction to protein expression and purification, and is committed to promoting the development of more new drugs for rare diseases.

Overview of Protein Expression and Purification

Protein expression is to transform the gene of the target protein into the expression vector and produce the target protein in the cell through transfection and transformation, to achieve the purpose of efficient protein purification and functional research. Commonly used expression systems include bacteria, yeast, insect cells, mammalian cells, etc. Protein expression and purification services have a wide range of applications in biomedical research, drug development, and other fields, providing researchers with a convenient way to obtain high-purity, high-quality proteins for further research and application.

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Fig.1 Protein expression workflow using baculovirus expression system as an example. (Tang, Yanyang, et al. 2020)

Our Services

Our company offers a complete range of recombinant protein expression solutions. We will customize our service based on your specific needs, and be responsible for project design, codon optimization, gene synthesis, and transfection.

Expression System	Service Content	Notes
*E. coli*	Plasmid construction Transformation and strain screening Target protein expression and purification Endotoxin removal Quality control	Rapid Most cost-effective Clear genetic background Extensive experience in purification from the inclusion body Endotoxin ctrl available upon request
Mammalian Cells	Plasmid construction Small-volume transfected cells Expanded cell culture Protein purification Quality control	High-density cell culture High expression level Endotoxin-free A collection of proprietary lead sequences and linkers
*B. subtilis*	Plasmid construction 16 protein conditions tested Small-scale protein production Mass production and purification Quality control	Rapid Achieve better protein folding Endotoxin-free Better production of IFN-β/γ and IFN-α2b
Yeast	Expression vector construction Transformation and strain identification Protein expression screening of 20-40 strains Protein purification Quality control	Endotoxin-free The product can be modified after translation High secretion Protein stability
Insect Cells	Plasmid construction Preparation of recombinant Bacmid and high titer virus Extended expression Protein purification Quality control	High success rate Usually used for large proteins and intracellular proteins Glycosylation Sf9 cells and High-five cells are available

Our company uses the advanced AKTA protein purification system to provide a variety of protein purification methods, in order to meet the different needs of customers, including but not limited to:

Affinity chromatography
Ion exchange chromatography

Hydrophobic interaction chromatography
Size exclusion chromatography

Our advantages

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Extensive experience in protein expression
Customized services and products tailored to your research
High data quality and reliable analysis
Fast and cost-efficient workflow
Timely project reporting and after-sales service

We are willing to work closely with our customers to develop the application of protein engineering in the field of rare diseases. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.

Reference

Tang, Yanyang, et al. "Construction of gateway-compatible baculovirus expression vectors for high-throughput protein expression and in vivo microcrystal screening." Scientific Reports 10.1 (2020): 13323.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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