Protein Immobilization Service
Immobilized proteins are specific proteins which are attached to a solid surface or medium, have longer active lifespans, are more stable, and thus are critical in the evolving domain of developing medicines for orphan diseases. Within the domain of specialization of our company, we offer a range of protein immobilization services which utilize our proprietary method of protein coupling.
Overview of Protein Immobilization
Protein immobilization is one of the most astonishing interdisciplinary scientific advances combining biotechnology, medicine, and engineering. It entails putting a target protein on a solid substrate surface either through porous medium or covalent bonding, making it highly useful in biochemical processes.
Fig.1 Overview of immobilized protein (Castner, David G., 2017)
Application of Protein Immobilization
- Drug delivery
The application of the protein immobilization service is multifaceted, especially in the delivery of therapeutics. For instance, drugs or growth factors can be covalently bound to polymeric particles or matrices and achieve prolonged release. Proteins that are cross-linked in those systems can serve not only as carriers but also as release controlling signal molecules to bring optimal effect. - Protein purification
An integral part of biopharmaceuticals is the purification of proteins. One can easily accomplish protein purification using a solid medium with specific affinity. - Enzyme catalyzed reaction
Reactions catalyzed through immobilized enzymes have also found wide application in biotransformation and industrial production. Effective and stable enzyme catalyzed reactions can be achieved using enzymes that are embedded or fixed in a solid media. It becomes easier as well as cost-effective to recycle and reuse these immobilized enzymes.
Our Services
Protein drugs are treated as polymers in order to achieve long-term controlled release. This helps maximize the efficacy of the drugs. The company offers an array of protein immobilization materials which utilize various substrates and functional groups. These systems are easily achieved using covalent coupling or immune binding techniques.
Protein covalent coupling
Our firm is capable of performing covalent protein coupling by modifying different chemical groups for coupling onto different matrix materials like PS matrix, Agarose matrix, and SiO2 matrix. The range of chemical modifications that can be done extend beyond these examples:
- NHS
- Aldehyde(-CHO)
- Carboxyl(-COOH)
- Amino(-NH2)
- Epoxy
- Tosyl
Protein immunobinding
Our firm is capable of achieving immune binding to a variety of target proteins by selectively modifying streptavidin, Protein A, Protein G, and GST on different nano materials like PS substrate, Agarose substrate, and SiO2. Some other examples of the Protein Immunobinding services we are able to provide are the following:
- SA magnetic bead
- Protein A/G magnetic beads
- GST magnetic beads
- Ni magnetic bead
Project Workflow
For many years, we keep working with scientific research institutions and universities to offer technology services of protein immunobinding at the professional level. We are quite open to collaborating with our clients to expand the scope of applications of immunobinding proteins in the area of rare diseases. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.
Reference
- Castner, David G. "Biomedical surface analysis: Evolution and future directions." Biointerphases 12.2 (2017).
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.