Single-stranded Circular DNA Therapy Development
Like other ssDNAs, single-stranded circular DNA (circDNA) possesses a covalently closed topology which makes it popular in the design of circDNA therapies. Our company has tailored solutions that stretch across the entire breadth of craniofacial gene therapy, ensuring that all potential hurdles and challenges in developing single-strand circular DNA therapies for orphan diseases are surmounted.
Overview of Single-stranded Circular DNA
Although single-stranded circular DNA (circDNA) is colloquially known as circular single-stranded DNA (cssDNA), it is tightly associated with viruses, bacteria, and eukaryotic mitochondria and chloroplasts. Single-stranded circular DNA generally has tighter structural constraints making it more stable than linear DNA and enables it to store more genetic information. Most DNA is found in a double helix form but is preferably in a single-stranded form when subject to heat or alkalis. Unlike double-stranded DNA, single stranded DNA differ from the former through the molecular hydrodynamic features, absorption spectra, and base reaction features.
Fig.1 Schematic depiction of single-stranded circDNA therapeutics and diagnostics (theranostics). (Shen, T., et al., 2022)
Materials for DNA-based therapies having low cost and biocompatibility, coupled with a simple chemical modification make them useful. Also, circDNA that lacks terminals is biologically more stable as it is less prone to exonuclease degradation. This makes circDNA useful in an array of DNA based therapies.
Applications of circDNA in DNA-based Therapy
circDNA Aptamers for Targeted Drug Delivery
The delivery of monomeric aptamers is compared to that of circDNA aptamers and it shows that the latter have better serum retention and intracellular delivery. circDNA aptamers can also serve to recognize homologous molecules for binding and targeting.
circDNA for Sensitive Bioanalysis
RCA is the only isothermal nucleic acid amplification that employs the synthesis of long single-stranded nucleic acids by continuous unidirectional replication of circular DNA templates. This makes bioanalysis greatly sensitive.
circDNA as Donors for CRISPR-Cas system
circDNA has low off-target integration rates, and high editing efficiency which makes it an ideal donor in gene editing. It is the most proficient tool of CRISPR-Cas system and can enhance the efficiency of gene knock-in, in vitro transcription, and many more experiments.
circDNA as Templates for Biomaterials Synthesis
circDNA can be used as a template for the synthesis of novel DNA biomaterials such as DNA nanoflower, DNA origami, and DNA hydrogel for a broad range of biomedical uses.
Our Services
We have the finest fully integrated platform that creates therapies for single stranded circular DNA using the proprietary biomarker detection methods. From our extensive knowledge, we are able to create therapies using single stranded isn't DNA for gene editing and targeting directly to the markers of rare diseases.
Single-stranded circular DNA Production
- Scalable bacteriophage production of isogenic cssDNA
- Shaker flask production of pure cssDNA
- Batch fermenter production of pure cssDNA
Single-stranded circular DNA Testing
- Agarose gel electrophoresis for purity testing
- Sanger sequencing for equence accuracy
- Spectrophotometer for optical density testing
Single-stranded circular DNA Delivery
Why Choose Us?
Our team prides itself upon the quality of service we provide to our clients. Hence this gives our company an edge. A clinician can utilize the development service of single-stranded circular DNA therapy for rare diseases. In the event that we are not meeting your expectations, we are ready to put in all the needed effort to guarantee that we meet your communication and problem solving needs. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.
Reference
- Shen, T., et al. "Single-stranded circular DNA theranostics." Theranostics 12.1 (2022): 35.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.