In developing therapies for skin diseases, especially rare ones, 3D bioprinting models have provided profound methods for the reproduction of intricate human skin structures. Protheragen develops advanced bioprinting models for accurate skin disease modeling, drug testing and therapy evaluation.
3D Bioprinting Models for Rare Skin Diseases
Rare skin diseases are notoriously difficult to study and treat but 3D bioprinting models represent a good solution to their challenges. Researchers can replicate the structure and functions of human skin more accurately by engineering realistic skin tissues through 3D printing. To make the models more representative of actual skin pathologies and more accurate than conventional 2D cultures, skin tissues are modeled with layered constructs of various cell types and extracellular matrix components. The creation of complex tissues for better understanding of the disease mechanisms, drug testing, as well as the treatment development for the rare skin diseases is made possible with 3D bioprinting.
Fig.1 Schematic representation of
in vitro HSE build up. (Zhang, M.,
et al., 2023)
3D Bioprinting Methods
The three main methods of 3D bioprinting, particularly used in skin tissue engineering, are:
Extrusion bioprinting
This technique uses a specific type of bioprinter that applies bioinks in continuous lines using a pneumatic pump or a mechanical piston system. It is characterized by high printing speed, low cost, high productivity, and the possibility of working with biomaterials of high viscosity. It is still prone to clogging of the nozzle and application of mechanical pressure during printing.
Inkjet-based biopritning
Bioprinting with inkjet technology works by dropwise deposition which can be done using thermal or piezoelectric effects. The advantages of this technique include low cost, high resolution, high cell viability, and non-contact printing. Limitations include a restricted choice of bioink, relatively weaker scaffolds, nozzle blockage, and mechanical or thermal stress on cells.
Laser-assisted bioprinting
Bioprinting Using Lasers (LAB) can eject droplets without using nozzles by creating bubbles in the bioink with a laser at the required locations. This approach is also non-contact, free of scopes, and very precise, as well as achieves very good cell viability. However, a tedious flow level because of fast gelation, low scaling, and being time intense continues to be an issue.
Our Services
Protheragen provides bioprinting processes for the development of 3D skin models necessary for the study of rare skin diseases.With our precise bioprinting instruments and our team's vast knowledge, Protheragen is able to develop, with high accuracy and innovation, 3D skin models that replicate the complex structure of the human skin.
Workflow of 3D Skin Bioprinting Models Development
Model Design & Bioink Preparation
Protheragen works with clients to design and produce customized 3D skin models and bioinks for optimal model creation based on cell type and biomaterial selection.
3D Bioprinting
Skin models are printed using the latest bioprinting machinery which allows us to produce skin models with precision on every layer of the model guaranteeing correct resolution and cell placement.
Maturation & Culturing
After the printing process, the models are put in controlled environments where cells will be able to differentiate and develop into fully functional tissues.
Characterization & Validation
The completed models are subjected to a series of validations including histology, gene expression, and human skin simulations for research purposes to determine their accuracy.
Types of 3D Skin Bioprinting Models
The creation of skin tissues for medical applications like burns, cosmetic testing, and solutions for wounds is possible with 3D skin bioprinting. Each of these applications has a defined purpose and each has a few distinct types of 3D skin bioprinting models.
- Full-Thickness Skin Models
- Dermal Models
- Skin Graft Models
- Hybrid Skin Models
- Biodegradable Skin Models
- Melanocyte-Containing Models
Why Choose Us?
Professional core technical team.
Advanced experimental equipment.
Empowering success through cooperation.
Strict quality control system.
At Protheragen, we remain committed to delivering in-depth and all-inclusive preclinical development services, primarily focusing on the 3D skin bioprinting model for an in-depth research on rare skin diseases. From diseases model development to drug safety evaluation, our services support every stage of your research process. If you are interested in our services, please don't hesitate to contact us.
References
- Olejnik, A., et al. "3d Bioprinting in Skin Related Research: Recent Achievements and Application Perspectives." ACS Synth Biol 11.1 (2022): 26-38.
- Zhang, M., et al. "Advances in 3d Skin Bioprinting for Wound Healing and Disease Modeling." Regen Biomater 10 (2023): rbac105.
All of our services and products are intended for preclinical research use
only and cannot be used to diagnose, treat or manage patients.
