Drug metabolism and pharmacokinetics is crucial for understanding drug penetration and distribution within the skin, serving as a key element in developing safe and effective therapies. Utilizing cutting-edge technologies and a team of seasoned experts, we expedite the analysis of drug behavior in the skin, delivering precise data that supports innovative treatment solutions for rare skin conditions.
Introduction to DMPK
DMPK is a core discipline in the drug discovery and development process, playing a crucial role in determining which drug candidates progress to clinical trials. By examining the ADME/T (Absorption, Distribution, Metabolism, Excretion, and Toxicity) properties and pharmacokinetics (PK) of a drug candidate, DMPK studies provide essential insights into the candidate's bioavailability, distribution, and potential interactions. Understanding drug metabolism, particularly the transformation by liver enzymes, is vital for predicting drug-drug interactions and ensuring the safety and efficacy of new therapeutics.
Fig.1 Cutaneous Pharmacokinetics of Topically Applied Novel Dermatological Formulations. (Patel, M.,
et al., 2024)
DMPK Evaluation of Rare Skin Diseases
By combining in vitro studies that analyze drug absorption and metabolism in skin models with in vivo pharmacokinetics in animal models, researchers gain essential insights into drug behavior, facilitating the creation of optimized therapies targeted at these challenging conditions.
In Vitro ADME properties:
- Evaluate drug absorption in reconstructed human epidermis or cultured skin cells to predict penetration efficiency in real-world applications.
- Use in vitro models to study the distribution of drugs across different skin layers, providing insights into local effects.
- Analyze metabolic pathways in ex vivo models to assess the metabolic stability and potential toxicity of drug candidates.
In Vivo Pharmacokinetics:
- Conduct studies in animal models to understand systemic absorption and skin retention, which helps infer human bioavailability.
- Measure concentrations of the drug in blood and skin tissues to evaluate distribution volume and potential therapeutic effects.
- Investigate the metabolic pathways and clearance rates to identify major metabolites and provide data for clinical dosing adjustments.
DMPK Evaluation of Rare Skin Diseases
Table1. Advantages and Disadvantages of Various Methods Used for the Determination of Cutaneous Pharmacokinetics. (Patel, M., et al., 2024)
| Method |
Advantages |
Disadvantages |
| Tape stripping |
Easily assess active moiety levels in the stratum corneum without biopsy-induced scarring or pain. |
Uneven tape attachment and individual epidermal differences may cause poor correlation, targeting primarily the stratum corneum rather than deeper skin layers. |
| Vasoconstrictor assay |
Serves as a pharmacodynamic endpoint for bioavailability assessment |
Only applicable for the corticosteroids formulations |
| Confocal Raman microscopy |
Characterization of skin in depth at different focal planes without physical cutting of tissue |
Limited fluorescent dyes and static images fail to capture the dynamic, long-term process of skin permeation. |
| MALDI-MSI |
High molecular specificity |
Time consuming; hence, it is a low throughput technique |
| Microfluidic devices |
Ensures rapid and accurate diagnosis with reduced reagent consumption. |
Complicated structure, hence required technical skill |
| Skin biopsy & Skin blistering |
Use to diagnose skin cancers and related diseases |
Difficulty in the analysis of lipophilic moiety |
Our Services
At our company, we are proud to provide specialized pharmacokinetic evaluation services tailored for rare skin diseases. Our skilled team of pharmacologists, toxicologists, and researchers utilizes state-of-the-art methodologies and profound expertise in the area to enhance the development and optimization of your therapeutic solutions.
In Vitro ADME Services
- Absorption Studies
- Distribution Analysis
- Metabolism Assessment
- Excretion Profiling
- Drug-Drug Interaction Evaluation
- ADME Property Profiling
In Vivo Pharmacokinetics Services
- Absorption Studies
- Tissue Distribution Analysis
- Metabolism Profiling
- Excretion Studies
- Bioavailability and Bioequivalence Testing
- Pharmacokinetic Parameter Analysis
Why Choose Us?
With a deep commitment to advancing treatments for rare skin diseases, our team combines scientific rigor with industry expertise to offer a streamlined, preclinical one-stop service. From drug discovery to preclinical pharmacokinetics, we provide tailored solutions designed to accelerate your therapeutic development process.
Professional core technical team.
Advanced experimental equipment.
Empowering success through cooperation.
Strict quality control system.
At our company, we are committed to providing comprehensive, one-stop preclinical development services that cover every aspect from disease model creation to innovative therapy research. If you are interested in our services, please don't hesitate to contact us.
References
- Eirefelt, S., et al. "Evaluating Dermal Pharmacokinetics and Pharmacodymanic Effect of Soft Topical Pde4 Inhibitors: Open Flow Microperfusion and Skin Biopsies." Pharm Res 37.12 (2020): 243.
- Patel, M., et al. "Cutaneous Pharmacokinetics of Topically Applied Novel Dermatological Formulations." AAPS PharmSciTech 25.3 (2024): 46.
All of our services and products are intended for preclinical research use
only and cannot be used to diagnose, treat or manage patients.
