Spheroid models offer a new highly innovative way to recreate the very sophisticated tissue structures of human skin in the context of rare skin disease research and development. Protheragen uses these models to deepen the understanding of the targeted diseases and speed up the creation of novel therapeutic cure which offers expert spheroid development services.
Introduction to Skin Spheroids
Skin spheroids are aggregates of skin cells cultured in a 3D format. They are generated from primary keratinocytes, fibroblasts, and melanoma cells which are usually grown with the hanging drop technique. Compared to traditional two-dimensional cell cultures, these models are more accurate for assessing drug efficacy, toxicity, and skin permeation technology. They are extensively used in biomedical research for skin diseases, like melanoma, as well as for primary screening of new drugs in conditions close to the clinic setting.
Fig.1 Genetically modified cell spheroids. (Jiang, Z.,
et al.,2023)
Three-Dimensional Skin Spheroid Cultures
Compared to standard 2D cultures, three-dimensional (3D) skin spheroid cultures are a great improvement because they provide greater detail on cell morphology, cellular interactions, matrix and even cellular responses. However, creating effective systems for primary human cells keratinocytes in 3D spheroid cultures is difficult.
Advantages
Compared to standard 2D cultures, 3D skin spheroid cultures are a great improvement because they provide greater detail on cell morphology, cellular interactions, matrix and even cellular responses. However, creating effective systems for primary human cells keratinocytes in 3D spheroid cultures is difficult.
Challenges
In comparison to classic 2D cultures, 3D suspension cultures of epithelial cells are easier to conduct and replicates an organism's anatomy lis more accurately. These systems are able to reconstruct great tissue features including cell structure architecture, cell-to-matrix interactions, and even osmotic exchange.
Our Services
Our promise at Protheragen is to drive forward research on uncommon skin diseases through the development of base breaking spheroid models. At Protheragen, we make accurate, dependable, and biologically realistic skin models utilizing advanced 3D cell culture technology for biological spheroid models. Our spheroid models are customized to the specific demands of our customers and clients to achieve maximum results.
Workflow of Spheroids Development
Consultation and Design
The requirements for research will be resolved and the appropriate spheroid models with the goal cell type, culture conditions and other specifications needed to accomplish your goals will be designed.
Spheroid Formation
Hanging drop and other advanced bioreactor methods are employed to promote the formation of spheroids by allowing the cells to agglomerate in three-dimensional structures which resemble human skin.
Customization and Optimization
Spheroids are augmented by specific factors, co-cultures, or other environmental conditions to increase the fidelity of the disease model or therapeutic target of choice.
Validation and Analysis
The spheroids are cross validated at histology, gene expression, and functional levels, with constant assistance and analysis support given the aim of successful completion of the research endeavor.
Types of Skin Spheroids
Skin spheroids are cultured skin tissues that are assembled in three dimension forming spheroids such as keratinocytes, fibroblasts, and melanocytes that recapitulate the natural structure and functions of skin tissue. These models are widely utilized in preclinical studies and studies in dermatology, cosmetics, and tissue engineering.
- Keratinocyte Spheroids
- Fibroblast Spheroids
- Full Skin Spheroids
- Melanocyte-Containing Spheroids
- 3D Skin Tumor Spheroids
- Stem Cell-Derived Skin Spheroids
Why Choose Protheragen?
- Expertise and Experience: Protheragen is a leader in rare skin disease modeling and therapeutic development. Assisted with years of experience in developing advanced skin models.
- Customized Solutions: We design and service targeted spheroid models and services to achieve the goals of your research with greater relevance and accuracy.
- Advanced Technologies: Protheragen utilizes modern innovations in 3D cell culturing and tissue engineering to develop high quality and physiologically relevant models.
- Comprehensive Support: From the first consultation to beyond initial research activities, we provide a complete package of services for your research to be effective and successful.
Protheragen offers a unique set of services for preclinical development through development of spheroid models for studies of rare skin diseases. From diseases model development to drug safety evaluation, our services support every stage of your research process. If you are interested in our services, please don't hesitate to contact us.
References
- Gionet-Gonzales, M. A., and J. K. Leach. "Engineering Principles for Guiding Spheroid Function in the Regeneration of Bone, Cartilage, and Skin." Biomed Mater 13.3 (2018): 034109.
- Jiang, Z., et al. "Genetically Modified Cell Spheroids for Tissue Engineering and Regenerative Medicine." J Control Release 354 (2023): 588-605.
All of our services and products are intended for preclinical research use
only and cannot be used to diagnose, treat or manage patients.
