Polyclonal Antibody Development Platform
Polyclonal antibodies are a heterogeneous mix of antibodies formed by a variety of B cells in the body. With a research team with extensive expertise in rare disease therapy research and development as well as an advanced therapeutic antibody development platform, our company is confident to provide customers with polyclonal antibody development services for rare diseases. We can provide one-stop personalized project management services according to customers' specific requirements.
Introduction to Polyclonal Antibody
Polyclonal antibodies are generally produced by immunizing a suitable mammal, such as a mouse, rabbit, or goat. If you want to collect more serum, you can use large mammals. First, specific antigens are injected into experimental animals to stimulate the host to produce a certain immune response and produce different types of antibodies in the blood. The antibodies stimulated by one antigen are called polyclonal antibodies. Animals are immunized secondary and tertiary to produce the highest titer of antibodies against the antigen. After a few weeks, polyclonal antibodies can then be collected and purified from the antisera.
Fig.1 Principle of polyclonal antibody preparation. (Mandal, A., et al., 2023)Polyclonal antibodies are widely used in modern biopharmaceutical fields because of their unique advantages:
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Our Services
With the world's top platform, in addition to the development of polyclonal antibodies, our company also provides preclinical research on polyclonal antibodies, including PK analysis and drug safety evaluation.
- Antigen Preparation
With its rich experience and complete recombinant protein expression platform, our company can provide efficient antigen preparation services to ensure the quality and quantity of antigens. - Adjuvant Selection and Preparation
Experienced scientists can select appropriate adjuvants to improve or enhance the immune response to the antigen and obtain high-quality antibodies. - Animal Immunization
Animals such as chickens, rabbits, goats, horses, guinea pigs, hamsters, rats, mice and sheep are available for polyclonal antibody production, with rats generally recommended.
Immunization protocol: Pre-immune bleed / Animal immunization / Serum titer test / Final bleed
Duration: Standard immunization: 9-12 weeks / Fast immunization: 5-6 weeks - Antibody Purification
Our company provides protein A/G affinity purification, antigen-specific affinity purification and other purification methods to remove impurities and non-specific proteins and isolate specific polyclonal antibodies from antiserum. Other optional purification methods include:
- Protein A purification
- Protein G purification
- Affinity purification
- IgY purification
- IgG purification
- Others
- Quality Control
To ensure the quality of polyclonal antibodies, our company provides you with quality control services to ensure the success rate of your downstream experiments.
Application validations: IHC, FC, ICC, ELISA, WB, Neutralization
Specificity validations: ELISA, Protein Microarray - Antibody Conjugation
Providing antibody conjugation services, including biotin, horseradish peroxidase (HRP), alkaline peroxidase, fluorescent dye/dye conjugation, to meet customer-specific needs.
Why Choose Us?
- Cutting-edge technology and equipment
- Providing standardized upstream platform solutions
- Competitive pricing and fast turnaround time
- One-stop platform with an experienced team
- Global reach and distribution network
- Superior data quality and fast turnaround
Project Workflow
As an integrated CRO, our company has extensive expertise in polyclonal antibody development for rare disease research. Whatever your specific requirements, we will work closely with you to develop a customized solution. We can quickly respond to the changing needs of your rare disease research projects. If you are interested in our services, please feel free to contact us for more details and quotation information of related services.
Reference
- Mandal, A., et al. "An insight into the agents used for immunomodulation and their mechanism of action." How Synthetic Drugs Work. Academic Press, 2023. 503-528.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.